Share Email Print
cover

Proceedings Paper

3D visualization and biovolume estimation of motile cells by digital holography
Author(s): F. Merola; L. Miccio; P. Memmolo; G. Di Caprio; G. Coppola; P. Netti
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

For the monitoring of biological samples, physical parameters such as size, shape and refractive index are of crucial importance. However, up to now the morphological in-vitro analysis of in-vitro cells has been limited to 2D analysis by classical optical microscopy such as phase-contrast or DIC. Here we show an approach that exploits the capability of optical tweezers to trap and put in self-rotation bovine spermatozoa flowing into a microfluidic channel. At same time, digital holographic microscopy allows to image the cell in phase-contrast modality for each different angular position, during the rotation. From the collected information about the cell’s phase-contrast signature, we demonstrate that it is possible to reconstruct the 3D shape of the cell and estimate its volume. The method can open new pathways for rapid measurement of in-vitro cells volume in microfluidic lab-on-a-chip platform, thus having access to 3D shape of the object avoiding tomography microscopy, that is an overwhelmed and very complex approach for measuring 3D shape and biovolume estimation.

Paper Details

Date Published: 8 May 2014
PDF: 7 pages
Proc. SPIE 9129, Biophotonics: Photonic Solutions for Better Health Care IV, 91291W (8 May 2014); doi: 10.1117/12.2052836
Show Author Affiliations
F. Merola, Istituto Nazionale di Ottica, CNR (Italy)
L. Miccio, Istituto Nazionale di Ottica, CNR (Italy)
P. Memmolo, Istituto Nazionale di Ottica, CNR (Italy)
Istituto Italiano di Tecnologia (Italy)
G. Di Caprio, Istituto per la Microelettronica e Microsistemi, CNR (Italy)
G. Coppola, Istituto per la Microelettronica e Microsistemi, CNR (Italy)
P. Netti, Istituto Italiano di Tecnologia (Italy)


Published in SPIE Proceedings Vol. 9129:
Biophotonics: Photonic Solutions for Better Health Care IV
Jürgen Popp; Valery V. Tuchin; Dennis L. Matthews; Francesco Saverio Pavone; Paul Garside, Editor(s)

© SPIE. Terms of Use
Back to Top