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Proceedings Paper

Raman tweezers on bacteria: following the mechanisms of bacteriostatic versus bactericidal action
Author(s): Silvie Bernatova; Ota Samek; Zdenek Pilat; Mojmir Sery; Jan Jezek; Petr Jakl; Martin Siler; Vladislav Krzyzanek; Pavel Zemanek; Veronika Hola; Milada Dvorackova; Filip Ruzicka
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Paper Abstract

Raman tweezers represents a unique method for identification of different microorganisms on the basis of Raman scattering. Raman tweezers allows us to fix and sterile manipulate with the trapped object and in the same time check the growth, viability, response to the external environment etc. by Raman signal evaluating. The investigations presented here include distinction of bacteria in general (staphylococcal cells), identification of bacteria strains (biofilm-positive and biofilm-negative) by using principal component analysis (PCA) and monitoring the influence of antibiotics.

Paper Details

Date Published: 8 May 2014
PDF: 7 pages
Proc. SPIE 9129, Biophotonics: Photonic Solutions for Better Health Care IV, 91291Y (8 May 2014); doi: 10.1117/12.2052538
Show Author Affiliations
Silvie Bernatova, Institute of Scientific Instruments of the ASCR v.v.i. (Czech Republic)
Ota Samek, Institute of Scientific Instruments of the ASCR v.v.i. (Czech Republic)
Zdenek Pilat, Institute of Scientific Instruments of the ASCR v.v.i. (Czech Republic)
Mojmir Sery, Institute of Scientific Instruments of the ASCR v.v.i. (Czech Republic)
Jan Jezek, Institute of Scientific Instruments of the ASCR v.v.i. (Czech Republic)
Petr Jakl, Institute of Scientific Instruments of the ASCR v.v.i. (Czech Republic)
Martin Siler, Institute of Scientific Instruments of the ASCR v.v.i. (Czech Republic)
Vladislav Krzyzanek, Institute of Scientific Instruments of the ASCR v.v.i. (Czech Republic)
Pavel Zemanek, Institute of Scientific Instruments of the ASCR v.v.i. (Czech Republic)
Veronika Hola, St. Anne's Faculty Hospital (Czech Republic)
Milada Dvorackova, St. Anne's Faculty Hospital (Czech Republic)
Filip Ruzicka, St. Anne's Faculty Hospital (Czech Republic)


Published in SPIE Proceedings Vol. 9129:
Biophotonics: Photonic Solutions for Better Health Care IV
Jürgen Popp; Valery V. Tuchin; Dennis L. Matthews; Francesco Saverio Pavone; Paul Garside, Editor(s)

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