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Proceedings Paper

Time-resolved imaging system for fluorescence-guided surgery with lifetime imaging capability
Author(s): F. Powolny; K. Homicsko; R. Sinisi; Claudio E. Bruschini; E. Grigoriev; H. Homulle; John O. Prior; D. Hanahan; E. Dubikovskaya; E. Charbon
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Paper Abstract

We present a single-photon camera for fluorescence imaging, with a time resolution better than 100ps, capable of providing both intensity and lifetime images. the camera was fabricated in standard CMOS technology. With this FluoCam we show the possibility to study sub-nanosecond fluorescence mechanisms. The FluoCam was used to characterize a near-infrared probe, indocyanine green, conjugated with multimeric cyclic pentapeptide (cRGD). The fluorescent probe-conjugated was used to target and mark tumors with better specificity, in particular aiming at targeting the integrins αvβ3 and αvβ5. As a first step towards clinical studies, preliminary results obtained in-vivo are presented. The first envisioned clinical application would be image-guided surgical oncology to help the surgeon to remove tumor tissue by a better discrimination from normal tissues and also to improve the detection of metastatic lymph nodes. A further application could be the in-vivo determination of the αvβ3 and αvβ5 targets to select patients for therapy with RGD chemotherapy conjugates.

Paper Details

Date Published: 8 May 2014
PDF: 6 pages
Proc. SPIE 9129, Biophotonics: Photonic Solutions for Better Health Care IV, 912938 (8 May 2014); doi: 10.1117/12.2052411
Show Author Affiliations
F. Powolny, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
K. Homicsko, Ctr. Hospitalier Univ. Vaudois (Switzerland)
Ecole Polytechnique Fédérale de Lausanne (Switzerland)
R. Sinisi, FORIMTECH S.A. (Switzerland)
Claudio E. Bruschini, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
E. Grigoriev, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
H. Homulle, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
John O. Prior, Ctr. Hospitalier Univ. Vaudois (Switzerland)
D. Hanahan, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
E. Dubikovskaya, FORIMTECH S.A. (Switzerland)
E. Charbon, Ecole Polytechnique Fédérale de Lausanne (Switzerland)
Technische Univ. Delft (Netherlands)


Published in SPIE Proceedings Vol. 9129:
Biophotonics: Photonic Solutions for Better Health Care IV
Jürgen Popp; Valery V. Tuchin; Dennis L. Matthews; Francesco Saverio Pavone; Paul Garside, Editor(s)

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