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Proceedings Paper

Measurement of the optical properties of rat brain tissue using contact spatially resolved spectroscopy
Author(s): Barbara Gysbrechts; Nghia Nguyen Do Trong; Ling Wang; Henrique Cabral; Zaneta Navratilova; Francesco P. Battaglia; Wouter Saeys; Carmen Bartic
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Paper Abstract

Nowadays, biophotonics is widely used in neuroscience. The effectiveness of biophotonic techniques, such as fluorescence imaging and optogenetics, is affected by the optical properties of the examined tissue. Therefore, knowledge of these properties is essential to carefully plan experiments. Mice and rats are widely used in neuroscience studies. However, reports about optical properties of their brains are very rare. We measured optical absorption μa and reduced scattering μ’s coefficients of native rat brain in the visible and near-infrared wavelength region, using contact spatially resolved spectroscopy (SRS). In this study, we estimate μa and μ’s for the rat cortex and discuss their stability in time. Additionally, variations in optical properties within and between samples were characterized. The results extend the range of known optical properties for the rat cortex, especially in the visible range, relevant to optogenetics. μa and μ’s are stable within a time span of four hours, and show low variation in and between brain samples. This indicates that a suitable protocol was used to estimate optical properties of rodent brain tissue. Since contact SRS is a non-destructive method, this technique could be used also to measure μa and μ’s in living animals. Moreover, the probe has small dimensions, allowing the characterization of optical properties in different structures of the brain.

Paper Details

Date Published: 8 May 2014
PDF: 7 pages
Proc. SPIE 9129, Biophotonics: Photonic Solutions for Better Health Care IV, 912936 (8 May 2014); doi: 10.1117/12.2052367
Show Author Affiliations
Barbara Gysbrechts, Katholieke Univ. Leuven (Belgium)
Nghia Nguyen Do Trong, Katholieke Univ. Leuven (Belgium)
Ling Wang, Katholieke Univ. Leuven (Belgium)
Henrique Cabral, Neuro-Electronics Research Flanders (Belgium)
Radboud Univ. Nijmegen (Netherlands)
Zaneta Navratilova, Neuro-Electronics Research Flanders (Belgium)
Radboud Univ. Nijmegen (Netherlands)
Francesco P. Battaglia, Neuro-Electronics Research Flanders (Belgium)
Radboud Univ. Nijmegen (Netherlands)
Wouter Saeys, Katholieke Univ. Leuven (Belgium)
Carmen Bartic, Katholieke Univ. Leuven (Belgium)

Published in SPIE Proceedings Vol. 9129:
Biophotonics: Photonic Solutions for Better Health Care IV
Jürgen Popp; Valery V. Tuchin; Dennis L. Matthews; Francesco Saverio Pavone; Paul Garside, Editor(s)

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