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Proceedings Paper

High speed microscopy techniques for signaling detection in live cells
Author(s): C. de Mauro; C. A. Cecchetti; D. Alfieri; Giulia Borile; A. Urbani; M. Mongillo; F. S. Pavone
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Paper Abstract

Alterations in intracellular cardiomyocyte calcium handling have a key role in initiating and sustaining arrhythmias. Arrhythmogenic calcium leak from sarcoplasmic reticulum (SR) can be attributed to all means by which calcium exits the SR store in an abnormal fashion. Abnormal SR calcium exit maymanifest as intracellular Ca2+ sparks and/or Ca2+ waves. Ca2+ signaling in arrhythmogenesis has been mainly studied in isolated cardiomyocytes and given that the extracellular matrix influences both Ca2+ and membrane potential dynamics in the intact heart and underlies environmentally mediated changes, understanding how Ca2+ and voltage are regulated in the intact heart will represent a tremendous advancement in the understanding of arrhythmogenic mechanisms. Using novel high-speed multiphoton microscopy techinques, such as multispot and random access, we investigated animal models with inherited and acquired arrhythmias to assess the role of Ca2+ and voltage signals as arrhythmia triggers in cell and subcellular components of the intact heart and correlate these with electrophysiology.

Paper Details

Date Published: 8 May 2014
PDF: 6 pages
Proc. SPIE 9129, Biophotonics: Photonic Solutions for Better Health Care IV, 91291J (8 May 2014); doi: 10.1117/12.2052177
Show Author Affiliations
C. de Mauro, Light4Tech Firenze S.r.l. (Italy)
C. A. Cecchetti, Light4Tech Firenze S.r.l. (Italy)
D. Alfieri, Light4Tech Firenze S.r.l. (Italy)
Giulia Borile, Venetian Institute of Molecular Medicine (Italy)
A. Urbani, Venetian Institute of Molecular Medicine (Italy)
M. Mongillo, Venetian Institute of Molecular Medicine (Italy)
F. S. Pavone, Univ. degli Studi di Firenze (Italy)


Published in SPIE Proceedings Vol. 9129:
Biophotonics: Photonic Solutions for Better Health Care IV
Jürgen Popp; Valery V. Tuchin; Dennis L. Matthews; Francesco Saverio Pavone; Paul Garside, Editor(s)

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