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Proceedings Paper

Fluorescence particle detection using microfluidics and planar optoelectronic elements
Author(s): Siegfried W. Kettlitz; Carola Moosmann; Sebastian Valouch; Uli Lemmer
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Paper Abstract

Detection of fluorescent particles is an integral part of flow cytometry for analysis of selectively stained cells. Established flow cytometer designs achieve great sensitivity and throughput but require bulky and expensive components which prohibit mass production of small single-use point-of-care devices. The use of a combination of innovative technologies such as roll-to-roll printed microuidics with integrated optoelectronic components such as printed organic light emitting diodes and printed organic photodiodes enables tremendous opportunities in cost reduction, miniaturization and new application areas. In order to harvest these benefits, the optical setup requires a redesign to eliminate the need for lenses, dichroic mirrors and lasers. We investigate the influence of geometric parameters on the performance of a thin planar design which uses a high power LED as planar light source and a PIN-photodiode as planar detector. Due to the lack of focusing optics and inferior optical filters, the device sensitivity is not yet on par with commercial state of the art flow cytometer setups. From noise measurements, electronic and optical considerations we deduce possible pathways of improving the device performance. We identify that the sensitivity is either limited by dark noise for very short apertures or by noise from background light for long apertures. We calculate the corresponding crossover length. For the device design we conclude that a low device thickness, low particle velocity and short aperture length are necessary to obtain optimal sensitivity.

Paper Details

Date Published: 8 May 2014
PDF: 8 pages
Proc. SPIE 9129, Biophotonics: Photonic Solutions for Better Health Care IV, 91293W (8 May 2014); doi: 10.1117/12.2052140
Show Author Affiliations
Siegfried W. Kettlitz, Karlsruher Institut für Technologie (Germany)
Carola Moosmann, Karlsruher Institut für Technologie (Germany)
Sebastian Valouch, Karlsruher Institut für Technologie (Germany)
Uli Lemmer, Karlsruher Institut für Technologie (Germany)


Published in SPIE Proceedings Vol. 9129:
Biophotonics: Photonic Solutions for Better Health Care IV
Jürgen Popp; Valery V. Tuchin; Dennis L. Matthews; Francesco Saverio Pavone; Paul Garside, Editor(s)

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