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Proceedings Paper

Direct imaging of singlet oxygen luminescence generated in blood vessels during photodynamic therapy
Author(s): Lisheng Lin; Huiyun Lin; Defu Chen; Longchao Chen; Min Wang; Shusen Xie; Ying Gu; Brian C. Wilson; Buhong Li
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Paper Abstract

Singlet oxygen (1O2) is commonly recognized to be a major phototoxic component for inducing the biological damage during photodynamic therapy (PDT). In this study, a novel configuration of a thermoelectrically-cooled near-infrared sensitive InGaAs camera was developed for imaging of photodynamically-generated 1O2 luminescence. The validation of 1O2 luminescence images for solution samples was performed with the model photosensitizer Rose Bengal (RB). Images of 1O2 luminescence generated in blood vessels in vivo in a well-controlled dorsal skinfold window chamber model were also recorded during PDT. This study demonstrated the capacity of the newly-developed imaging system for imaging of 1O2 luminescence, and the first reported images of 1O2 luminescence in blood vessels in vivo. This system has potential for elucidating the mechanisms of vascular targeted PDT.

Paper Details

Date Published: 8 May 2014
PDF: 7 pages
Proc. SPIE 9129, Biophotonics: Photonic Solutions for Better Health Care IV, 912920 (8 May 2014); doi: 10.1117/12.2052033
Show Author Affiliations
Lisheng Lin, Fujian Normal Univ. (China)
Huiyun Lin, Fujian Normal Univ. (China)
Defu Chen, Fujian Normal Univ. (China)
Chinese PLA General Hospital (China)
Longchao Chen, Fujian Normal Unv. (China)
Min Wang, Fujian Normal Univ. (China)
Shusen Xie, Fujian Normal Univ. (China)
Ying Gu, Chinese PLA General Hospital (China)
Brian C. Wilson, Princess Margaret Cancer Ctr., Univ. of Toronto (Canada)
Buhong Li, Fujian Normal Univ. (China)

Published in SPIE Proceedings Vol. 9129:
Biophotonics: Photonic Solutions for Better Health Care IV
Jürgen Popp; Valery V. Tuchin; Dennis L. Matthews; Francesco Saverio Pavone; Paul Garside, Editor(s)

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