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Proceedings Paper

Porphyrin involvement in redshift fluorescence in dentin decay
Author(s): A. Slimani; I. Panayotov; B. Levallois; T. Cloitre; C. Gergely; N. Bec; C. Larroque; H. Tassery; F. Cuisinier
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Paper Abstract

The aim of this study was to evaluate the porphyrin involvement in the red fluorescence observed in dental caries with Soprolife® light-induced fluorescence camera in treatments mode (SOPRO, ACTEON Group, La Ciotat, France) and Vistacam® camera (DÜRR DENTAL AG, Bietigheim-Bissingen, Germany). The International Caries Detection and Assessment System (ICDAS) was used to rand the samples. Human teeth cross-sections, ranked from ICDAS score 0 to 6, were examined by epi-fluorescence microscopy and Confocal Raman microscopy. Comparable studies were done with Protoporphyrin IX, Porphyrin I and Pentosidine solutions. An RGB analysis of Soprolife® images was performed using ImageJ Software (1.46r, National Institutes of Health, USA). Fluorescence spectroscopy and MicroRaman spectroscopy revealed the presence of Protoporphyrin IX, in carious enamel, dentin and dental plaque. However, the presence of porphyrin I and pentosidine cannot be excluded. The results indicated that not only porphyrin were implicated in the red fluorescence, Advanced Glygation Endproducts (AGEs) of the Maillard reaction also contributed to this phenomenon.

Paper Details

Date Published: 8 May 2014
PDF: 8 pages
Proc. SPIE 9129, Biophotonics: Photonic Solutions for Better Health Care IV, 91291C (8 May 2014); doi: 10.1117/12.2051741
Show Author Affiliations
A. Slimani, Lab. Nanoscience et Biosanté, Univ. Montpellier 1 (France)
I. Panayotov, Lab. Nanoscience et Biosanté, Univ. Montpellier 1 (France)
B. Levallois, Lab. Nanoscience et Biosanté, Univ. Montpellier 1 (France)
T. Cloitre, Lab. Charles Coulomb, Univ. Montpellier 2 (France)
C. Gergely, Lab. Charles Coulomb, Univ. Montpellier 2 (France)
N. Bec, INSERM, Institut de Recherche en Cancérologie de Montpellier (France)
C. Larroque, INSERM, Institut de Recherche en Cancérologie de Montpellier (France)
H. Tassery, Lab. Nanoscience et Biosanté, Univ. Montpellier 1 (France)
Aix-Marseille-Univ. (France)
F. Cuisinier, Lab. Nanoscience et Biosanté, Univ. Montpellier 1 (France)


Published in SPIE Proceedings Vol. 9129:
Biophotonics: Photonic Solutions for Better Health Care IV
Jürgen Popp; Valery V. Tuchin; Dennis L. Matthews; Francesco Saverio Pavone; Paul Garside, Editor(s)

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