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Proceedings Paper

Zinc oxide nanostructures for electrochemical cortisol biosensing
Author(s): Phani Kiran Vabbina; Ajeet Kaushik; Kathryn Tracy; Shekhar Bhansali; Nezih Pala
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Paper Abstract

In this paper, we report on fabrication of a label free, highly sensitive and selective electrochemical cortisol immunosensors using one dimensional (1D) ZnO nanorods (ZnO-NRs) and two dimensional nanoflakes (ZnO-NFs) as immobilizing matrix. The synthesized ZnO nanostructures (NSs) were characterized using scanning electron microscopy (SEM), selective area diffraction (SAED) and photoluminescence spectra (PL) which showed that both ZnO-NRs and ZnO-NFs are single crystalline and oriented in [0001] direction. Anti-cortisol antibody (Anti-Cab) are used as primary capture antibodies to detect cortisol using electrochemical impedance spectroscopy (EIS). The charge transfer resistance increases linearly with increase in cortisol concentration and exhibits a sensitivity of 3.078 KΩ. M-1 for ZnO-NRs and 540 Ω. M -1 for ZnO-NFs. The developed ZnO-NSs based immunosensor is capable of detecting cortisol at 1 pM. The observed sensing parameters are in physiological range. The developed sensors can be integrated with microfluidic system and miniaturized potentiostat to detect cortisol at point-of-care.

Paper Details

Date Published: 22 May 2014
PDF: 8 pages
Proc. SPIE 9107, Smart Biomedical and Physiological Sensor Technology XI, 91070U (22 May 2014); doi: 10.1117/12.2051156
Show Author Affiliations
Phani Kiran Vabbina, Florida International Univ. (United States)
Ajeet Kaushik, Florida International Univ. (United States)
Kathryn Tracy, Florida International Univ. (United States)
Shekhar Bhansali, Florida International Univ. (United States)
Nezih Pala, Florida International Univ. (United States)

Published in SPIE Proceedings Vol. 9107:
Smart Biomedical and Physiological Sensor Technology XI
Brian M. Cullum; Eric S. McLamore, Editor(s)

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