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Proceedings Paper

FRET based characterization of DNA-based assemblies
Author(s): Susan Buckhout-White; Rochester Gray; Mario Ancona; Ellen R. Goldman; Igor L. Medintz
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Paper Abstract

The “spectroscopic ruler” based on fluorescence resonance energy transfer (FRET) is explored as a method for detailed structural characterization of DNA nanostructures in solution. The approach is most directly useful for assessing the positional relationships among chromophores organized by the DNA, but it can also be used to characterize the geometry and kinematics of the DNA scaffold itself. By accumulating data for the distances separating various donor-acceptor pairs, and correlating them with the expected distances, one can quantify the shape and deformability of the structure. A 8x16nm “mini-origami” rectangle is used as the model test structure and the dye-pairs are chosen to investigate anisotropy in the origami’s mechanical properties. Not unexpectedly, our analysis finds a strong anisotropy in the stiffness, with the measured spacing across the origami weave deviating much more from expectation than the spacing aligned along the weave pattern.

Paper Details

Date Published: 28 May 2014
PDF: 8 pages
Proc. SPIE 9107, Smart Biomedical and Physiological Sensor Technology XI, 91070Y (28 May 2014); doi: 10.1117/12.2050923
Show Author Affiliations
Susan Buckhout-White, George Mason Univ. (United States)
U.S. Naval Research Lab. (United States)
Rochester Gray, U.S. Naval Research Lab. (United States)
Mario Ancona, U.S. Naval Research Lab. (United States)
Ellen R. Goldman, U.S. Naval Research Lab. (United States)
Igor L. Medintz, U.S. Naval Research Lab. (United States)

Published in SPIE Proceedings Vol. 9107:
Smart Biomedical and Physiological Sensor Technology XI
Brian M. Cullum; Eric S. McLamore, Editor(s)

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