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Proceedings Paper

Self-referencing digital holographic microscope for dynamic imaging of living cells
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Paper Abstract

Digital holographic microscope is an ideal tool for quantitative phase contrast imaging of living cells. It yields the thickness distribution of the object under investigation from a single hologram. From a series of holograms the dynamics of the cell under investigation can be obtained. But two-beam digital holographic microscopes has low temporal stability due to uncorrelated phase changes occurring in the reference and object arms. One way to overcome is to use common path techniques, in which, the reference beam is derived from the object beam itself. Both the beams travel along the same path, increasing the temporal stability of the setup. In self-referencing techniques a portion of the object beam is converted into reference beam. It could be achieved by example, using a glass plate to create two laterally sheared versions of the object beam at the sensor, which interfere to produce the holograms/interferograms. This created a common path setup, leading to high temporal stability (~0.6nm). This technique could be used to map cell membrane fluctuations with high temporal stability. Here we provide an overview of our work on the development of temporally stable quantitative phase contrast techniques for dynamic imaging of micro-objects and biological specimen including red blood cells.

Paper Details

Date Published: 5 June 2014
PDF: 8 pages
Proc. SPIE 9117, Three-Dimensional Imaging, Visualization, and Display 2014, 91170X (5 June 2014); doi: 10.1117/12.2050415
Show Author Affiliations
Arun Anand, The Maharaja Sayajirao Univ. of Baroda (India)
Vani Chhaniwal, The Maharaja Sayajirao Univ. of Baroda (India)
Swapnil Mahajan, The Maharaja Sayajirao Univ. of Baroda (India)
Vismay Trivedi, The Maharaja Sayajirao Univ. of Baroda (India)
Amardeep Singh, Medizinische Univ. Wien (Austria)
Rainer Leitgeb, Medizinische Univ. Wien (Austria)
Bahram Javidi, Univ. of Connecticut (United States)

Published in SPIE Proceedings Vol. 9117:
Three-Dimensional Imaging, Visualization, and Display 2014
Bahram Javidi; Jung-Young Son; Osamu Matoba; Manuel Martínez-Corral; Adrian Stern, Editor(s)

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