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Proceedings Paper

Single gold nanoparticles to enhance the detection of single fluorescent molecules at micromolar concentration using fluorescence correlation spectroscopy
Author(s): Deep Punj; Hervé Rigneault; Jérôme Wenger
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Paper Abstract

Single nanoparticles made of noble metals are strongly appealing to develop practical applications to detect fluorescent molecules in solution. Here, we detail the use of a single gold nanoparticle of 100 nm diameter to enhance the detection of single Alex Fluor 647 fluorescent molecules at high concentrations of several micromolar. We discuss the implementation of fluorescence correlation spectroscopy, and provide a new method to reliably extract the enhanced fluorescence signal stemming from the nanoparticle near-field from the background generated in the confocal volume. The applicability of our method is checked by reporting the invariance of the single molecule results as function of the molecular concentration, and the experimental data is found in good agreement with numerical simulations.

Paper Details

Date Published: 2 May 2014
PDF: 9 pages
Proc. SPIE 9126, Nanophotonics V, 91261N (2 May 2014); doi: 10.1117/12.2049910
Show Author Affiliations
Deep Punj, Institut Fresnel, CNRS, Aix Marseille Univ. (France)
Hervé Rigneault, Institut Fresnel, CNRS, Aix Marseille Univ. (France)
Jérôme Wenger, Institut Fresnel, CNRS, Aix Marseille Univ. (France)


Published in SPIE Proceedings Vol. 9126:
Nanophotonics V
David L. Andrews; Jean-Michel Nunzi; Andreas Ostendorf, Editor(s)

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