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Proceedings Paper

Lab-on-a-chip modules for detection of highly pathogenic bacteria: from sample preparation to detection
Author(s): S. Julich; R. Kopinč; N. Hlawatsch; C. Moche; A. Lapanje; C. Gärtner; H. Tomaso
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Paper Abstract

Lab-on-a-chip systems are innovative tools for the detection and identification of microbial pathogens in human and veterinary medicine. The major advantages are small sample volume and a compact design. Several fluidic modules have been developed to transform analytical procedures into miniaturized scale including sampling, sample preparation, target enrichment, and detection procedures. We present evaluation data for single modules that will be integrated in a chip system for the detection of pathogens. A microfluidic chip for purification of nucleic acids was established for cell lysis using magnetic beads. This assay was evaluated with spiked environmental aerosol and swab samples. Bacillus thuringiensis was used as simulant for Bacillus anthracis, which is closely related but non-pathogenic for humans. Stationary PCR and a flow-through PCR chip module were investigated for specific detection of six highly pathogenic bacteria. The conventional PCR assays could be transferred into miniaturized scale using the same temperature/time profile. We could demonstrate that the microfluidic chip modules are suitable for the respective purposes and are promising tools for the detection of bacterial pathogens. Future developments will focus on the integration of these separate modules to an entire lab-on-a-chip system.

Paper Details

Date Published: 22 May 2014
PDF: 10 pages
Proc. SPIE 9107, Smart Biomedical and Physiological Sensor Technology XI, 91070R (22 May 2014); doi: 10.1117/12.2049859
Show Author Affiliations
S. Julich, Friedrich-Loeffler-Institut (Germany)
R. Kopinč, Institute of Microbial Sciences and Technologies (Slovenia)
Institute of Metagenomics and Microbial Technologies (Slovenia)
N. Hlawatsch, microfluidic ChipShop GmbH (Germany)
C. Moche, microfluidic ChipShop GmbH (Germany)
A. Lapanje, Institute of Microbial Sciences and Technologies (Slovenia)
C. Gärtner, microfluidic ChipShop GmbH (Germany)
H. Tomaso, Friedrich-Loeffler-Institut (Germany)

Published in SPIE Proceedings Vol. 9107:
Smart Biomedical and Physiological Sensor Technology XI
Brian M. Cullum; Eric S. McLamore, Editor(s)

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