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Proceedings Paper

Optical clearing of human skin for the enhancement of optical imaging of proximal interphalangeal joints
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Paper Abstract

We are proposing a new method for enhancement of optical imaging of proximal interphalangeal (PIP) joints in humans at skin using optical clearing technique. A set of illuminating laser diodes with the wavelengths 670, 820, and 904 nm were used as a light source. The laser diodes, monochromatic digital CCD camera and specific software allowed for detection of the finger joint image in a transillumination mode. The experiments were carried out in vivo with human fingers. Dehydrated glycerol and hand cream with urea (5%) were used as optical clearing agents (OCAs). The contrast of the obtained images was analyzed to determine the effect of the OCA. It was found that glycerol application to the human skin during 60 min caused the decrease of contrast in 1.4 folds for 670 nm and the increase of contrast in 1.5 and 1.7 folds for 820 nm and 904 nm, respectively. At the same time, the hand cream application to the human skin during 60 min caused the decrease of contrast in 1.1 folds for 670 nm and the increase of contrast in 1.3 and 1.1 folds for 820 nm and 904 nm, respectively. The results have shown that glycerol and the hand cream with 5% urea allow for obtaining of more distinct image of finger joint in the NIR. Obtained data can be used for development of optical diagnostic methods of rheumatoid arthritis.

Paper Details

Date Published: 30 January 2014
PDF: 10 pages
Proc. SPIE 9031, Saratov Fall Meeting 2013: Optical Technologies in Biophysics and Medicine XV; and Laser Physics and Photonics XV, 90310C (30 January 2014); doi: 10.1117/12.2049525
Show Author Affiliations
Ekaterina A. Kolesnikova, N.G. Chernyshevsky Saratov State Univ. (Russian Federation)
Aleksandr S. Kolesnikov, N.G. Chernyshevsky Saratov State Univ. (Russian Federation)
Urszula Zabarylo, Charité – Universitätsmedizin Berlin (Germany)
Olaf Minet, Charité – Universitätsmedizin Berlin (Germany)
Elina A. Genina, N.G. Chernyshevsky Saratov State Univ. (Russian Federation)
Alexey N. Bashkatov, N.G. Chernyshevsky Saratov State Univ. (Russian Federation)
Valery V. Tuchin, N.G. Chernyshevsky Saratov State Univ. (Russian Federation)
Institute of Precise Mechanics and Control (Russian Federation)
Univ. of Oulu (Finland)


Published in SPIE Proceedings Vol. 9031:
Saratov Fall Meeting 2013: Optical Technologies in Biophysics and Medicine XV; and Laser Physics and Photonics XV
Elina A. Genina; Vladimir L. Derbov; Igor Meglinski; Valery V. Tuchin, Editor(s)

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