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Proceedings Paper

Different matrix evaluation for the bone regeneration of rats' femours using time domain optical coherence tomography
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Paper Abstract

The osteoconductive materials are important in bone regeneration procedures. Three dimensional (3D) reconstructions were obtained from the analysis. The aim of this study is to investigate the interface between the femur rat bone and the new bone that is obtained using a method of tissue engineering that is based on two artificial matrixes inserted in previously artificially induced defects. For this study, under strict supervision 20 rats were used in conformity with ethical procedures. In all the femurs a round defect was induced by drilling with a 1 mm spherical Co-Cr surgical drill. The matrixes used were IngeniOss (for ten samples) and 4Bone(for the other ten samples). These materials were inserted into the induced defects. The femurs were investigated at 1 month, after the surgical procedures. The interfaces were examined using Time Domain (TD) Optical Coherence Tomography (OCT) combined with Confocal Microscopy (CM). The scanning procedure is similar to that used in any CM, where the fast scanning is en-face (line rate) and the scanning in depth is much slower (at the frame rate). The optical configuration uses two single mode directional couplers with a superluminiscent diode as the source centered at 1300 nm. The results showed open interfaces due to the insufficient healing process, as well as closed interfaces due to a new bone formation inside the defect. The conclusion of this study is that TD-OCT can act as a valuable tool in the investigation of the interface between the old bone and the one that has been newly created due to the osteoinductive process. The TD-OCT has proven a valuable tool for the non-invasive evaluation of the matrix bone interfaces.

Paper Details

Date Published: 14 January 2014
PDF: 5 pages
Proc. SPIE 8925, Fifth International Conference on Lasers in Medicine: Biotechnologies Integrated in Daily Medicine, 89250V (14 January 2014); doi: 10.1117/12.2045849
Show Author Affiliations
Laura-Cristina Rusu, Univ. of Medicine and Pharmacy Victor Babes Timisoara (Romania)
Meda Lavinia Negrutiu, Univ. of Medicine and Pharmacy Victor Babes Timisoara (Romania)
Cosmin Sinescu, Univ. of Medicine and Pharmacy Victor Babes Timisoara (Romania)
Bogdan Hoinoiu, Univ. of Medicine and Pharmacy Victor Babes Timisoara (Romania)
Cristian Zaharia, Univ. of Medicine and Pharmacy Victor Babes Timisoara (Romania)
Lavinia Ardelean, Univ. of Medicine and Pharmacy Victor Babes Timisoara (Romania)
Virgil-Florin Duma, 3OM Optomechatronics Group, Aurel Vlaicu Univ. of Arad (Romania)
Adrian Gh. Podoleanu, Univ. of Kent (United Kingdom)


Published in SPIE Proceedings Vol. 8925:
Fifth International Conference on Lasers in Medicine: Biotechnologies Integrated in Daily Medicine
Carmen Todea; Adrian Gh. Podoleanu; Virgil-Florin Duma, Editor(s)

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