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Proceedings Paper

Some unique features of alkaliphilic anaerobes
Author(s): Erin Roof; Elena Pikuta; Christopher Otto; George Williams; Richard Hoover
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Paper Abstract

This article explores two topics involving the examination of four strains of alkaliphilic anaerobes. The first topic was dedicated to detection of the ability of microorganisms to metabolize alternative chirality substrates. Two saccharolytic anaerobic bacteria were chosen for the first experiment: Anaerovirgula multivorans strain SCAT, which is gram positive and spore-forming; and Spirochaeta dissipatitropha, strain ASpC2T, which is gram negative. It was found that both checked sugarlytics were able to use L-ribose and L-arabinose, as growth substrates. The second part was concerned of study a chemolithotrophy in two halo-alkaliphilic sulfate reducing bacteria: Desulfonatornum thiodismutans strain MLF1T and Desulfonatronum lacustre strain Z-7951T. The experiments with lithotrophs had demonstrated that strain MLF1T was capable to grow without any organic source of carbon, while strain Z-7951T had required at least 2 mM sodium acetate for growth. Anaerobic technique was used for preparation of the growth media and maintenance of these bacterial cultures. Standard methods for Gram, spore, and flagella staining were applied for characterization of cytomorphology. In this article, the results of the experiments performed on cytological, physiological, and biochemical levels are presented and discussed.

Paper Details

Date Published: 26 September 2013
PDF: 12 pages
Proc. SPIE 8865, Instruments, Methods, and Missions for Astrobiology XVI, 88650F (26 September 2013); doi: 10.1117/12.2045350
Show Author Affiliations
Erin Roof, Athens State Univ. (United States)
Elena Pikuta, Athens State Univ. (United States)
Christopher Otto, Athens State Univ. (United States)
George Williams, Athens State Univ. (United States)
Richard Hoover, Athens State Univ. (United States)

Published in SPIE Proceedings Vol. 8865:
Instruments, Methods, and Missions for Astrobiology XVI
Richard B. Hoover; Gilbert V. Levin; Alexei Yu. Rozanov; Nalin C. Wickramasinghe, Editor(s)

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