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Proceedings Paper

Simultaneous real-time quantification of blood flow and vascular growth in the chick embryo using optical coherence tomography
Author(s): William J. Kowalski; Nikola C. Teslovich; Chia-Yuan Chen; Bradley B. Keller; Kerem Pekkan
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Paper Abstract

Experimental and clinical data indicate that hemodynamic forces within the embryo provide critical biomechanical cues for cardiovascular morphogenesis, growth, and remodeling and that perturbed flow is a major etiology of congenital heart disease. However, embryonic flow-growth relationships are largely qualitative and poorly defined. In this work, we provide a quantitative analysis of in vivo flow and growth trends in the chick embryo using optical coherence tomography (OCT) to acquire simultaneous velocity and structural data of the right vitelline artery continuously over a ten hour period beginning at stage 16 (hour 54). We obtained 3D vessel volumes (15 μm lateral, 4.3 μm axial resolutions, 6 μm slice spacing) at 60 minute intervals, taking a B-scan time series totaling one cardiac cycle at each slice. Embryos were maintained at a constant 37°C and 60% humidity during the entire acquisition period through an inhouse built chamber. The 3D vessel lumen geometries were reconstructed manually to assess growth. Blood flow velocity was computed from the central B-scan using red blood cell particle image velocimetry. The use of extended OCT imaging as a non-invasive method for continuous and simultaneous flow and structural data can enhance our understanding of the biomechanical regulation of critical events in morphogenesis. Data acquired will be useful to validate predictive finite-element 3D growth models.

Paper Details

Date Published: 4 March 2014
PDF: 11 pages
Proc. SPIE 8953, Optical Methods in Developmental Biology II, 895307 (4 March 2014); doi: 10.1117/12.2044932
Show Author Affiliations
William J. Kowalski, Carnegie Mellon Univ. (United States)
Nikola C. Teslovich, Carnegie Mellon Univ. (United States)
Chia-Yuan Chen, National Taiwan Univ. of Science and Technology (Taiwan)
Bradley B. Keller, Univ. of Louisville (United States)
Kerem Pekkan, Carnegie Mellon Univ. (United States)

Published in SPIE Proceedings Vol. 8953:
Optical Methods in Developmental Biology II
Andrew M. Rollins; Cecilia W. Lo; Scott E. Fraser, Editor(s)

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