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Proceedings Paper

Oxygen sensing glucose biosensors based on alginate nano-micro systems
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Paper Abstract

Clinically glucose monitoring in diabetes management is done by point-measurement. However, an accurate, continuous glucose monitoring, and minimally invasive method is desirable. The research aims at developing fluorescence-mediated glucose detecting biosensors based on near-infrared radiation (NIR) oxygen sensitive dyes. Biosensors based on Glucose oxidase (GOx)-Rudpp loaded alginate microspheres (GRAM) and GOx-Platinum-octaethylporphyrin (PtOEP)-PLAalginate microsphere system (GPAM) were developed using air-driven atomization and characterized using optical microscopy, CLSM, fluorescence spectro-photometry etc. Biosensing studies were performed by exposing standard solutions of glucose. Uniform sized GRAM and GPAM with size 50±10μm were formed using atomization. CLSM imaging of biosensors suggests that Rudpp and PtOEP nanoparticles are uniformly distributed in alginate microspheres. The GRAM and GPAM showed a good regression constant of 0.974 and of 0.9648 over a range of 0-10 mM of glucose with a high sensitivity of 3.349%/mM (625 nm) and 2.38%/mM (645 nm) at 10 mM of glucose for GRAM and GPAM biosensor. GRAM and GPAM biosensors show great potential in development of an accurate and minimally invasive glucose biosensor. NIR dye based assays can aid sensitive, minimally-invasive and interference-free detection of glucose in diabetic patients.

Paper Details

Date Published: 16 April 2014
PDF: 6 pages
Proc. SPIE 9060, Nanosensors, Biosensors, and Info-Tech Sensors and Systems 2014, 906005 (16 April 2014); doi: 10.1117/12.2044894
Show Author Affiliations
Rashmi Chaudhari, Indian Institute of Technology Bombay (India)
Abhijeet Joshi, Indian Institute of Technology Bombay (India)
Rohit Srivastava, Indian Institute of Technology Bombay (India)


Published in SPIE Proceedings Vol. 9060:
Nanosensors, Biosensors, and Info-Tech Sensors and Systems 2014
Vijay K. Varadan, Editor(s)

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