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Proceedings Paper

Wavelength-dependent measurement of contrast in NIR and extended NIR spectral range (650-1600 nm) in phantoms
Author(s): Daniel Salo; David M. Kim; Mikhail Y. Berezin
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Paper Abstract

In order to identify the optimal imaging conditions for the highest contrast in biological tissue, we explored the optical contrast of a phantom as a function of depth and wavelengths of excitation. Our customized optical hardware featured a scanning microscope, and imaging spectrographs equipped with silicon and InGaAs CCD diode array detectors allowed directed comparison of the intensity of NIR (650-900 nm) and exNIR (1000-1600 nm) light transmitted through a phantom (milk). We demonstrated that the contrast depends on the phantom thickness and the wavelength. At low depths (less than 3 mm) NIR light provides the best contrast while exNIR light shows significantly higher contrast in phantoms thicker than 4.5 mm. Our results suggest that distinguishing biological features in deep tissue may benefit from the application of the exNIR for in vivo.

Paper Details

Date Published: 17 March 2014
PDF: 7 pages
Proc. SPIE 8940, Optical Biopsy XII, 89400O (17 March 2014); doi: 10.1117/12.2044872
Show Author Affiliations
Daniel Salo, Washington Univ. School of Medicine in St. Louis (United States)
David M. Kim, Washington Univ. School of Medicine in St. Louis (United States)
Mikhail Y. Berezin, Washington Univ. School of Medicine in St. Louis (United States)

Published in SPIE Proceedings Vol. 8940:
Optical Biopsy XII
Robert R. Alfano; Stavros G. Demos, Editor(s)

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