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Proceedings Paper

Comparison of the efficiency control of mycotoxins by some optical immune biosensors
Author(s): N. F. Slyshyk; N. F. Starodub
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Paper Abstract

It was compared the efficiency of patulin control at the application of such optical biosensors which were based on the surface plasmon resonance (SPR) and nano-porous silicon (sNPS). In last case the intensity of the immune reaction was registered by measuring level of chemiluminescence (ChL) or photocurrent of nPS. The sensitivity of this mycotoxin determination by first type of immune biosensor was 0.05-10 mg/L Approximately the same sensitivity as well as the overall time analysis were demonstrated by the immune biosensor based on the nPS too. Nevertheless, the last type of biosensor was simpler in technical aspect and the cost of analysis was cheapest. That is why, it was recommend the nPS based immune biosensor for wide screening application and SPR one for some additional control or verification of preliminary obtained results. In this article a special attention was given to condition of sample preparation for analysis, in particular, micotoxin extraction from potao and some juices. Moreover, it was compared the efficiency of the above mentioned immune biosensors with such traditional approach of mycotoxin determination as the ELISA-method. In the result of investigation and discussion of obtained data it was concluded that both type of the immune biosensors are able to fulfill modern practice demand in respect sensitivity, rapidity, simplicity and cheapness of analysis.

Paper Details

Date Published: 18 November 2013
PDF: 5 pages
Proc. SPIE 9032, Biophotonics—Riga 2013, 903210 (18 November 2013); doi: 10.1117/12.2044756
Show Author Affiliations
N. F. Slyshyk, National Univ. of Life and Environmental Sciences of Ukraine (Ukraine)
N. F. Starodub, National Univ. of Life and Environmental Sciences of Ukraine (Ukraine)

Published in SPIE Proceedings Vol. 9032:
Biophotonics—Riga 2013
Janis Spigulis; Ilona Kuzmina, Editor(s)

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