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Proceedings Paper

Fabrication and characterization of a 3-D non-homogeneous tissue-like mouse phantom for optical imaging
Author(s): Stella Avtzi; Athanasios Zacharopoulos; Stylianos Psycharakis; Giannis Zacharakis
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Paper Abstract

In vivo optical imaging of biological tissue not only requires the development of new theoretical models and experimental procedures, but also the design and construction of realistic tissue-mimicking phantoms. However, most of the phantoms available currently in literature or the market, have either simple geometrical shapes (cubes, slabs, cylinders) or when realistic in shape they use homogeneous approximations of the tissue or animal under investigation. The goal of this study is to develop a non-homogeneous realistic phantom that matches the anatomical geometry and optical characteristics of the mouse head in the visible and near-infrared spectral range. The fabrication of the phantom consisted of three stages. Initially, anatomical information extracted from either mouse head atlases or structural imaging modalities (MRI, XCT) was used to design a digital phantom comprising of the three main layers of the mouse head; the brain, skull and skin. Based on that, initial prototypes were manufactured by using accurate 3D printing, allowing complex objects to be built layer by layer with sub-millimeter resolution. During the second stage the fabrication of individual molds was performed by embedding the prototypes into a rubber-like silicone mixture. In the final stage the detailed phantom was constructed by loading the molds with epoxy resin of controlled optical properties. The optical properties of the resin were regulated by using appropriate quantities of India ink and intralipid. The final phantom consisted of 3 layers, each one with different absorption and scattering coefficient (μas) to simulate the region of the mouse brain, skull and skin.

Paper Details

Date Published: 18 November 2013
PDF: 6 pages
Proc. SPIE 9032, Biophotonics—Riga 2013, 903206 (18 November 2013); doi: 10.1117/12.2044698
Show Author Affiliations
Stella Avtzi, Foundation for Research and Technology-Hellas (Greece)
Athanasios Zacharopoulos, Foundation for Research and Technology-Hellas (Greece)
Stylianos Psycharakis, Foundation for Research and Technology-Hellas (Greece)
Giannis Zacharakis, Foundation for Research and Technology-Hellas (Greece)

Published in SPIE Proceedings Vol. 9032:
Biophotonics—Riga 2013
Janis Spigulis; Ilona Kuzmina, Editor(s)

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