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Proceedings Paper

Immune biosensors based on the SPR and TIRE: efficiency of their application for bacteria determination
Author(s): N. F. Starodub; J. Ogorodniichuk; T. Lebedeva; P. Shpylovyy
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Paper Abstract

In this work we have designed high-specific biosensors for Salmonella typhimurium detection based on the surface plasmon resonance (SPR) and total internal reflection ellipsometry (TIRE). It has been demonstrated high selectivity and sensitivity of analysis. As a registering part for our experiments the Spreeta (USA) and “Plasmonotest” (Ukraine) with flowing cell have been applied among of SPR device. Previous researches confirmed an efficiency of SPR biosensors using for detecting of specific antigen-antibody interactions therefore this type of reactions with some previous preparations of surface binding layer was used as reactive part. It has been defined that in case with Spreeta sensitivity was on the level 103 – 107 cells/ml. Another biosensor based on the SPR has shown the sensitivity within 101 – 106 cells/ml. Maximal sensitivity was on the level of several cells in 10 ml (up to the fact that less than 5 cells) which has been obtained using the biosensor based on TIRE.

Paper Details

Date Published: 18 November 2013
PDF: 4 pages
Proc. SPIE 9032, Biophotonics—Riga 2013, 90320X (18 November 2013); doi: 10.1117/12.2044648
Show Author Affiliations
N. F. Starodub, National Univ. of Life and Environmental Sciences of Ukraine (Ukraine)
J. Ogorodniichuk, National Univ. of Life and Environmental Sciences of Ukraine (Ukraine)
T. Lebedeva, V.M. Glushkov Institute of Cybernetics (Ukraine)
P. Shpylovyy, V.M. Glushkov Institute of Cybernetics (Ukraine)


Published in SPIE Proceedings Vol. 9032:
Biophotonics—Riga 2013
Janis Spigulis; Ilona Kuzmina, Editor(s)

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