Share Email Print
cover

Proceedings Paper

Adaptive optics two photon microscopy with direct wavefront sensing using autofluorescent guide-stars
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

A fast direct wavefront sensing method for dynamic in-vivo adaptive optical two photon microscopy has demonstrated. By using the direct wavefront sensing and open loop control, the system provides high-speed wavefront measurement and correction. To measure the wavefront in the middle of a Drosophila embryo at early stages, autofluorescence from endogenous fluorophores in the yolk were used as reference guide-stars. This method does not rely on fluorescently labeled proteins as guide-stars, which can simplify the sample preparation for wavefront measurement. The method was tested through live imaging of a Drosophila embryo. The aberration in the middle of the embryo was measured directly for the first time. After correction, both contrast and signal intensity of the structure in the middle of the embryo was improved.

Paper Details

Date Published: 7 March 2014
PDF: 6 pages
Proc. SPIE 8978, MEMS Adaptive Optics VIII, 89780D (7 March 2014); doi: 10.1117/12.2044090
Show Author Affiliations
Xiaodong Tao, Univ. of California, Santa Cruz (United States)
Andrew Norton, Univ. of California, Santa Cruz (United States)
Matthew Kissel, Univ. of California, Santa Cruz (United States)
Oscar Azucena, Univ. of California, Santa Cruz (United States)
Joel Kubby, Univ. of California, Santa Cruz (United States)


Published in SPIE Proceedings Vol. 8978:
MEMS Adaptive Optics VIII
Thomas G. Bifano; Joel Kubby; Sylvain Gigan, Editor(s)

© SPIE. Terms of Use
Back to Top