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Proceedings Paper

3D shape analysis of heterochromatin foci based on a 3D spherical harmonics intensity model
Author(s): Simon Eck; Stefan Wörz; Katharina Müller-Ott; Matthias Hahn; Gunnar Schotta; Karsten Rippe; Karl Rohr
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Paper Abstract

We propose a novel approach for 3D shape analysis of heterochromatin foci in 3D confocal light microscopy images of cell nuclei. The approach is based on a 3D parametric intensity model and uses a spherical harmonics (SH) expansion. The model parameters including the SH coefficients are automatically determined by least squares fitting of the model to the image intensities. Based on the obtained SH coefficients, a shape descriptor is determined, which enables distinguishing heterochromatin foci based on their 3D shape to characterize compaction states of heterochromatin. Our approach has been successfully applied to real static and dynamic 3D microscopy image data.

Paper Details

Date Published: 21 March 2014
PDF: 6 pages
Proc. SPIE 9034, Medical Imaging 2014: Image Processing, 90340X (21 March 2014); doi: 10.1117/12.2043482
Show Author Affiliations
Simon Eck, Univ. of Heidelberg (Germany)
German Cancer Research Ctr. (Germany)
Stefan Wörz, Univ. of Heidelberg (Germany)
German Cancer Research Ctr. (Germany)
Katharina Müller-Ott, Univ. of Heidelberg (Germany)
German Cancer Research Ctr. (Germany)
Matthias Hahn, Ludwig-Maximilians-Univ. München (Germany)
Gunnar Schotta, Ludwig-Maximilians-Univ. München (Germany)
Karsten Rippe, Univ. of Heidelberg (Germany)
German Cancer Research Ctr. (Germany)
Karl Rohr, Univ. of Heidelberg (Germany)
German Cancer Research Ctr. (Germany)


Published in SPIE Proceedings Vol. 9034:
Medical Imaging 2014: Image Processing
Sebastien Ourselin; Martin A. Styner, Editor(s)

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