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Proceedings Paper

Noncytotoxic Mn-doped ZnSe/ZnS quantum dots for biomedical applications
Author(s): John B. Plumley; Brian A. Akins; Gema J. Alas; Madalyn E. Fetrow; Jane Nguyen; Priyanka Jain; Stephanie Yang; Yekaterina I. Brandt; Gennady A. Smolyakov; Wojciech Ornatowski; Erin D. Milligan; Marek Osiński
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Paper Abstract

Quantum dots (QDs) are of high interest in the biomedical field. The most widely used and commercially available CdSe/ZnS QDs have a highly toxic Cd component. High-efficiency luminescent Cd-free Mn-doped ZnSe/ZnS QDs are a reasonable alternative to CdSe/ZnS QDs; however the actual cytotoxicity of ZnSe:Mn/ZnS QDs is relatively unknown. In this study, we apply the ApoTox-GloTM Triplex assay to test for cell cytotoxicity, viability, and induced apoptosis, by treating macrophage cells with different concentrations of peptide-coated ZnSe:Mn/ZnS QDs at four different incubation times: 6, 12, 24, and 48 hours. At the concentrations used, which varied between 0.03 μM to 0.25 μM, the macrophage cells showed very little cytotoxic effect. However, cell viability began to decrease with increasing QD concentration beginning with the 12 hour incubation time, with fairly consistent results for 24 and 48 hour incubation times as well. Also, the macrophage cells expressed a measurable degree of induced apoptosis, which scaled with concentration. While cytotoxicity did not seem to be an issue with macrophage cells treated with the peptide-coated Mn-doped ZnSe/ZnS QDs, the drop in cell viability and the increase in induced apoptosis suggest an antiproliferation effect within the macrophage cell culture.

Paper Details

Date Published: 24 March 2014
PDF: 11 pages
Proc. SPIE 8955, Colloidal Nanoparticles for Biomedical Applications IX, 895513 (24 March 2014); doi: 10.1117/12.2043293
Show Author Affiliations
John B. Plumley, The Univ. of New Mexico (United States)
Brian A. Akins, The Univ. of New Mexico (United States)
Gema J. Alas, The Univ. of New Mexico (United States)
Volcano Vista High School (United States)
Madalyn E. Fetrow, The Univ. of New Mexico (United States)
Volcano Vista High School (United States)
Jane Nguyen, The Univ. of New Mexico (United States)
Volcano Vista High School (United States)
Priyanka Jain, The Univ. of New Mexico (United States)
La Cueva High School (United States)
Stephanie Yang, The Univ. of New Mexico (United States)
Albuquerque Academy (United States)
Yekaterina I. Brandt, The Univ. of New Mexico (United States)
Gennady A. Smolyakov, The Univ. of New Mexico (United States)
Wojciech Ornatowski, UNM Health Sciences Ctr., The Univ. of New Mexico (United States)
Erin D. Milligan, UNM Health Sciences Ctr., The Univ. of New Mexico (United States)
Marek Osiński, The Univ. of New Mexico (United States)


Published in SPIE Proceedings Vol. 8955:
Colloidal Nanoparticles for Biomedical Applications IX
Wolfgang J. Parak; Marek Osinski; Kenji I. Yamamoto, Editor(s)

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