Share Email Print
cover

Proceedings Paper

Novel T lymphocyte proliferation assessment using whole mouse cryo-imaging
Author(s): Patiwet Wuttisarnwattana; Syed A. Raza; Saada Eid; Kenneth R. Cooke; David L. Wilson
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

New imaging technologies enable one to assess T-cell proliferation, an important feature of the immunological response. However, none of the traditional imaging modalities allow one to examine quantiatively T-cell function with microscopic resolution and single cell sensitivity over an entire mouse. To address this need, we established T-cells proliferation assays using 3D microscopic cryo-imaging. Assays include: (1) biodistribution of T-cells, (2) secondary lymphoid organ (SLO) volume measurement, (3) carboxyfluorescein succinimidyl ester (CFSE) dilution per cell as cells divide. To demonstrate the application, a graft-versus-host-disease (GVHD) model was used. 3D visualization show that T-cells specifically homed to the SLOs (spleen and lymph nodes) as well as GVHD target organs (such as GI-tract, liver, skin and thymus).The spleen was chosen as representative of the SLOs. For spleen size analysis, volumes of red and white pulp were measured. Spleen volumes of the allogeneic mice (with GVHD) were significantly larger than those of the syngeneic mice (without GVHD) at 72 to 120 hours post-transplant. For CFSE dilution approach, we employed color-coded volume rendering and probability density function (PDF) of single cell intensity to assess T-cell proliferation in the spleen. As compared to syngeneic T-cells, the allogeneic T-cells quickly aggregated in the spleen as indicated by increasing of CFSE signal over the first 48 hours. Then they rapidly proliferated as evidenced by reduced CFSE intensity (at 48-96 hours). Results suggest that assays can be used to study GVHD treatments using T-cell proliferation and biodistibution as assays. In summary, this is the first time that we are able to track and visualize T-cells in whole mouse with single cell sensitivity. We believe that our technique can be an alternative choice to traditional in vitro immunological proliferation assays by providing assessment of proliferation in an in vivo model.

Paper Details

Date Published: 13 March 2014
PDF: 9 pages
Proc. SPIE 9038, Medical Imaging 2014: Biomedical Applications in Molecular, Structural, and Functional Imaging, 90381U (13 March 2014); doi: 10.1117/12.2042960
Show Author Affiliations
Patiwet Wuttisarnwattana, Case Western Reserve Univ. (United States)
Syed A. Raza, COMSATS Institute of Information Technology (Pakistan)
Saada Eid, National Ctr. for Stem Cell & Regenerative Medicine (United States)
Kenneth R. Cooke, Johns Hopkins Univ. (United States)
David L. Wilson, Case Western Reserve Univ. (United States)
Univ. Hospitals, Cleveland (United States)
BioInVision Inc. (United States)


Published in SPIE Proceedings Vol. 9038:
Medical Imaging 2014: Biomedical Applications in Molecular, Structural, and Functional Imaging
Robert C. Molthen; John B. Weaver, Editor(s)

© SPIE. Terms of Use
Back to Top