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Proceedings Paper

Enhancing stimulated emission-based fluorescence detection with interferometric setup
Author(s): Shen-Shou Max Chung; Jia-Hui Deng; Po-Lin Lin; Fu-Jen Kao
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Paper Abstract

Fluorescence lifetime imaging microscopy (FLIM) can reveal important biological information and recently stimulated emission (SE) has been applied in FLIM to improve the spatial resolution of micrographs and detect fluorophore over a long working distance. An issue with SE is that the SE signal is much weaker than the probe laser beam that is used to generate the SE, therefore the signal to background ratio is low. Here we demonstrate using interferometric setup to decrease this background laser intensity, thus achieving higher S/N ratio and dye concentration detection sensitivity in SE microscopy.

Paper Details

Date Published: 28 February 2014
PDF: 9 pages
Proc. SPIE 8948, Multiphoton Microscopy in the Biomedical Sciences XIV, 89481T (28 February 2014); doi: 10.1117/12.2042085
Show Author Affiliations
Shen-Shou Max Chung, National Yang-Ming Univ. (Taiwan)
Jia-Hui Deng, National Yang-Ming Univ. (Taiwan)
Po-Lin Lin, National Yang-Ming Univ. (Taiwan)
Fu-Jen Kao, National Yang-Ming Univ. (Taiwan)

Published in SPIE Proceedings Vol. 8948:
Multiphoton Microscopy in the Biomedical Sciences XIV
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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