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Proceedings Paper

Photoirradiation study of gold nanospheres and rods in Vero and Hela cell lines
Author(s): Poorani Gananathan; Prakasarao Aruna; Singaravelu Ganesan; Manickan Elanchezhiyan
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Paper Abstract

Photoirradiation effect of gold nanospheres in conjucation with green light and rods in conjugation with red light corresponds to their absorption wavelength range found to be appreciable. In this present work concentration of nanomaterial and light dose were optimized. Gold nanospheres were synthesized by reduction technique using Sodium Borohydrate as reducing agent and Trisodium Citrate as capping agent. Au nanorods having 680-900nm absorption were synthesized using reduction techniques with CTAB and BDAC polymers. From UV-Vis absorption and Transmission Electron Microscopy the size of nanoparticles were confirmed. 30nm Gold nanospheres and green light source of 530nm wavelength with power 30mW were applied to Vero and Hela cell lines shows higher toxicity for Hela cells. Nanorods were applied and irradiated with 680nm wavelength light source with light intensity 45mW. Post irradiation effect for 24hrs, 48hrs confirms cell proliferation in normal rate in viable cells. The morphological changes in irradiated spot leads to apoptotoic cell death was confirmed with microscopic imaging. The LD50 value was also calculated.

Paper Details

Date Published: 24 March 2014
PDF: 6 pages
Proc. SPIE 8955, Colloidal Nanoparticles for Biomedical Applications IX, 89551T (24 March 2014); doi: 10.1117/12.2041933
Show Author Affiliations
Poorani Gananathan, Anna Univ. Chennai (India)
Prakasarao Aruna, Anna Univ. Chennai (India)
Singaravelu Ganesan, Anna Univ. Chennai (India)
Manickan Elanchezhiyan, Univ. of Madras Taramani Campus (India)

Published in SPIE Proceedings Vol. 8955:
Colloidal Nanoparticles for Biomedical Applications IX
Wolfgang J. Parak; Marek Osinski; Kenji I. Yamamoto, Editor(s)

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