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Proceedings Paper

Pulse splitter-based nonlinear microscopy for live-cardiomyocyte imaging
Author(s): Zhonghai Wang; Wan Qin; Yonghong Shao; Siyu Ma; Thomas K. Borg; Bruce Z. Gao
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Paper Abstract

Second harmonic generation (SHG) microscopy is a new imaging technique used in sarcomeric-addition studies. However, during the early stage of cell culture in which sarcomeric additions occur, the neonatal cardiomyocytes that we have been working with are very sensitive to photodamage, the resulting high rate of cell death prevents systematic study of sarcomeric addition using a conventional SHG system. To address this challenge, we introduced use of the pulse-splitter system developed by Na Ji et al. in our two photon excitation fluorescence (TPEF) and SHG hybrid microscope. The system dramatically reduced photodamage to neonatal cardiomyocytes in early stages of culture, greatly increasing cell viability. Thus continuous imaging of live cardiomyocytes was achieved with a stronger laser and for a longer period than has been reported in the literature. The pulse splitter-based TPEF-SHG microscope constructed in this study was demonstrated to be an ideal imaging system for sarcomeric addition-related investigations of neonatal cardiomyocytes in early stages of culture.

Paper Details

Date Published: 28 February 2014
PDF: 6 pages
Proc. SPIE 8948, Multiphoton Microscopy in the Biomedical Sciences XIV, 89482X (28 February 2014); doi: 10.1117/12.2041845
Show Author Affiliations
Zhonghai Wang, Clemson Univ. (United States)
Wan Qin, Clemson Univ. (United States)
Yonghong Shao, Shenzhen Univ. (China)
Siyu Ma, Clemson Univ. (United States)
Thomas K. Borg, Medical Univ. of South Carolina (United States)
Bruce Z. Gao, Clemson Univ. (United States)

Published in SPIE Proceedings Vol. 8948:
Multiphoton Microscopy in the Biomedical Sciences XIV
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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