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Proceedings Paper

Photochemical internalization (PCI) enhanced nonviral transfection of tumor suppressor and pro-drug activating genes; a potential treatment modality for gliomas
Author(s): Frederick Wang; Genesis Zamora; Chung-Ho Sun; Anthony Trinidad; Kristian Berg; Steen Madsen; Young Jik Kwon; Henry Hirschberg
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Paper Abstract

The overall objective of the research is to investigate the utility of photochemical internalization for the enhanced nonviral transfection of genes into cells. We have examined, in detail, the evaluation of photochemical internalization (PCI) as a method for the non-viral introduction of the tumor suppressor gene PTEN and the PCI mediated transfection of the cytosine deaminase (CD) pro drug activating gene into glioma cell monolayers and multi-cell tumor spheroids. Expression of the CD gene within the target cell produces an enzyme that converts the nontoxic prodrug, 5-fluorocytosine (5-FC), to the toxic metabolite, 5-fluorouracil (5-FU).

Paper Details

Date Published: 5 March 2014
PDF: 12 pages
Proc. SPIE 8928, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics, 89280B (5 March 2014); doi: 10.1117/12.2041627
Show Author Affiliations
Frederick Wang, Beckman Laser Institute and Medical Clinic (United States)
Genesis Zamora, Beckman Laser Institute and Medical Clinic (United States)
Chung-Ho Sun, Beckman Laser Institute and Medical Clinic (United States)
Anthony Trinidad, Beckman Laser Institute and Medical Clinic (United States)
Kristian Berg, Oslo Univ. Hospital (Norway)
Steen Madsen, Univ. of Nevada, Las Vegas (United States)
Young Jik Kwon, Univ. of California, Irvine (United States)
Henry Hirschberg, Beckman Laser Institute and Medical Clinic (United States)
Univ. of Nevada, Las Vegas (United States)


Published in SPIE Proceedings Vol. 8928:
Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics
Henry Hirschberg; E. Duco Jansen; Samarendra K. Mohanty; Nitish V. Thakor; Qingming Luo; Steen J. Madsen, Editor(s)

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