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Proceedings Paper

Irradiation at 660 nm modulates different genes central to wound healing in wounded and diabetic wounded cell models
Author(s): Nicolette N. Houreld
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Paper Abstract

Wound healing is a highly orchestrated process and involves a wide variety of cellular components, chemokines and growth factors. Laser irradiation has influenced gene expression and release of various growth factors, cytokines and extracellular matrix proteins involved in wound healing. This study aimed to determine the expression profile of genes involved in wound healing in wounded and diabetic wounded fibroblast cells in response to irradiation at a wavelength of 660 nm. Human skin fibroblast cells (WS1) were irradiated with a diode laser (wavelength 660 nm; fluence 5 J/cm2; power output 100 mW; power density 11 mW/cm2; spot size 9.1 cm2; exposure duration 7 min 35 s). Total RNA was isolated and 1 μg reverse transcribed into cDNA which was used as a template in real-time qualitative polymerase chain reaction (qPCR). Eighty four genes involved in wound healing (extracellular matrix and cell adhesion; inflammatory cytokines and chemokines; growth factors; and signal transduction) were evaluated in wounded and diabetic wounded cell models. Forty eight hours post-irradiation, 6 genes were significantly upregulated and 8 genes were down-regulated in irradiated wounded cells, whereas 1 gene was up-regulated and 33 genes down-regulated in irradiated diabetic wounded cells. Irradiation of stressed fibroblast cells to a wavelength of 660 nm and a fluence of 5 J/cm2 modulated the expression of different genes involved in wound healing in different cell models. Modulation of these genes leads to the effects of laser irradiation seen both in vivo and in vitro, and facilitates the wound healing process.

Paper Details

Date Published: 18 February 2014
PDF: 8 pages
Proc. SPIE 8932, Mechanisms for Low-Light Therapy IX, 89320A (18 February 2014); doi: 10.1117/12.2041430
Show Author Affiliations
Nicolette N. Houreld, Univ. of Johannesburg (South Africa)

Published in SPIE Proceedings Vol. 8932:
Mechanisms for Low-Light Therapy IX
Michael R. Hamblin; James D. Carroll; Praveen Arany, Editor(s)

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