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Proceedings Paper

Dental pulp stem cells (DPSCs) differentiation study by confocal Raman microscopy
Author(s): H. Salehi; P.-Y. Collart-Dutilleul; C. Gergely; F. J. G. Cuisinier
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Paper Abstract

Regenerative medicine brings a huge application for Mesenchymal stem cells such as Dental Pulp Stem Cells (DPSCs). Confocal Raman microscopy, a non-invasive, label free , real time and high spatial resolution imaging technique is used to study osteogenic differentiation of DPSCs. Integrated Raman intensities in the 2800-3000 cm-1 region (C-H stretching) and 960 cm-1 peak (phosphate PO4 3-) were collected. In Dental Pulp Stem Cells 21st day differentiated in buffer solution, phosphate peaks ν1 PO4 3- (first vibrational mode) at 960cm-1 and ν2 PO4 3- at 430cm–1 and ν4 PO4 3- at 585cm-1 are obviously present. Confocal Raman microscopy enables the detection of cell differentiation and it can be used to investigate clinical stem cell research.

Paper Details

Date Published: 4 March 2014
PDF: 10 pages
Proc. SPIE 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, 89470O (4 March 2014); doi: 10.1117/12.2041346
Show Author Affiliations
H. Salehi, Lab. Biologie-Santé Nanosciences, Univ. Montpellier 1 (France)
P.-Y. Collart-Dutilleul, Lab. Biologie-Santé Nanosciences, Univ. Montpellier 1 (France)
C. Gergely, Univ. Montpellier 2, Lab. Charles Coulomb, CNRS (France)
F. J. G. Cuisinier, Lab. Biologie-Santé Nanosciences, Univ. Montpellier 1 (France)


Published in SPIE Proceedings Vol. 8947:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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