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Proceedings Paper

Alterations in cell migration and cell viability of wounded human skin fibroblasts following visible red light exposure
Author(s): Vijendra Prabhu; Bola Sadashiva Satish Rao; Krishna Kishore Mahato
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Paper Abstract

The present study intended to examine the effect of visible red light on structural and cellular parameters on wounded skin fibroblast cells. To achieve the stated objective, uniform scratch was created on confluent monolayered human skin fibroblast cells, and were exposed to single dose of He-Ne laser (15 mm spot, 6.6808 mWcm-2) at 1, 2, 3, 4, 5, 6 and 7 Jcm-2 in the presence and absence of 10% fetal bovine serum (FBS). Beam profile measurements of the expanded laser beam were conducted to ensure the beam uniformity. The influence of laser dose on the change in temperature was recorded using sensitive temperature probe. Additionally, following laser exposure cell migration and cell survival were documented at different time intervals on wounded human skin fibroblast cells grown in vitro. Beam profile measurements indicated more or less uniform power distribution over the whole beam area. Temperature monitoring of sham irradiated control and laser treatment groups displayed negligible temperature change indicating the absence of thermal effect at the tested laser doses. In the absence of 10% FBS, single exposure of different laser doses failed to produce any significant effects on cell migration or cell survival. However, in the presence of serum single exposure of 5 J/cm2 on wounded skin fibroblasts significantly enhanced the cell migration (P<0.05) compared to the other tested doses (1, 2, 3, 4, 6 and 7 J/cm2) and sham irradiated controls. In conclusion, the LLLT acts by improving cell migration and cell proliferation to produce measurable changes in wounded fibroblast cells.

Paper Details

Date Published: 18 February 2014
PDF: 7 pages
Proc. SPIE 8932, Mechanisms for Low-Light Therapy IX, 89320C (18 February 2014); doi: 10.1117/12.2041174
Show Author Affiliations
Vijendra Prabhu, Manipal Univ. (India)
Bola Sadashiva Satish Rao, Manipal Univ. (India)
Krishna Kishore Mahato, Manipal Univ. (India)


Published in SPIE Proceedings Vol. 8932:
Mechanisms for Low-Light Therapy IX
Michael R. Hamblin; James D. Carroll; Praveen Arany, Editor(s)

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