Share Email Print
cover

Proceedings Paper

Flexibly combined optical microangiography and dual-wavelength laser speckle system for comprehensive imaging of hemodynamic and metabolic responses
Author(s): Lei Shi; Jia Qin; Lin An; Ruikang K. Wang
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

We have proposed and developed a multi-modal non-invasive biomedical optical imager. It was combined from the subsystems of optical microangiography and dual-wavelength laser speckle contrast imaging. The system was designed to maintain the performances of both subsystems. It was capable of simultaneously imaging the hemodynamic and metabolic responses in tissue environment in vivo. To achieve such requirements, we utilized unique optical setup, such as paired dichroic mirrors to compensate dispersion, additional relay lens to increase working distance and translational sample probe to freely select imaging area and focal plane. The multi-functionality of the system was demonstrated in an investigation of hemodynamic and metabolic responses on an acute wound healing model in mouse pinna in vivo. The microvasculature, blood flow and hemoglobin concentration from millimeter down to capillary level were comprehensively visualized. The captured instantaneous responses to wound onset differed greatly between localized areas; after that blood flow had a rebalance tendency, and hemoglobin concentration dynamically recovered to baseline situation.

Paper Details

Date Published: 4 March 2014
PDF: 6 pages
Proc. SPIE 8934, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVIII, 89341F (4 March 2014); doi: 10.1117/12.2041152
Show Author Affiliations
Lei Shi, Univ. of Washington (United States)
Jia Qin, Univ. of Washington (United States)
Lin An, Univ. of Washington (United States)
Ruikang K. Wang, Univ. of Washington (United States)


Published in SPIE Proceedings Vol. 8934:
Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XVIII
Joseph A. Izatt; James G. Fujimoto; Valery V. Tuchin, Editor(s)

© SPIE. Terms of Use
Back to Top