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Proceedings Paper

Imaging marine virus CroV and its host Cafeteria roenbergensis with two-photon microscopy
Author(s): Bin Cao; Sayan Chakraborty; Wenqing Sun; Seyedmohammadali Aghvami; Matthias G. Fischer; Wei Qian; Chuan Xiao; Chunqiang Li
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Paper Abstract

We use two-photon microscopy to monitor the infection process of marine zooplankton, Cafeteria roenbergensis (C.roenbergensis), by Cafeteria roenbergensis virus (CroV), a giant DNA virus named after its host. Here, we image C.roenbergensis in culture by two-photon excited NADH autofluorescence at video-rate (30 frame/s), and the movement of C.roenbergensis is recorded in live videos. Moreover, CroV is stained with DNA dye SYBR gold and recorded simultaneously with this two-photon microscope. We observed the initial infection moment with this method. The result demonstrates the potential use of two-photon microscopy to investigate the fast dynamic interaction between C.roenbergensis with virus CroV. After catching this initial moment, we will freeze the sample in liquid nitrogen for cryo-electron microscopy (EM) study to resolve the virus-host interaction at molecular level. The long-term goal is to study similar fast moving pathogen-host interaction process which could lead to important medical applications.

Paper Details

Date Published: 27 February 2014
PDF: 6 pages
Proc. SPIE 8944, Biophotonics and Immune Responses IX, 89440E (27 February 2014); doi: 10.1117/12.2041121
Show Author Affiliations
Bin Cao, The Univ. of Texas at El Paso (United States)
Sayan Chakraborty, The Univ. of Texas at El Paso (United States)
Wenqing Sun, The Univ. of Texas at El Paso (United States)
Seyedmohammadali Aghvami, The Univ. of Texas at El Paso (United States)
Matthias G. Fischer, Max Planck Institute for Medical Research (Germany)
Wei Qian, The Univ. of Texas at El Paso (United States)
Chuan Xiao, The Univ. of Texas at El Paso (United States)
Chunqiang Li, The Univ. of Texas at El Paso (United States)

Published in SPIE Proceedings Vol. 8944:
Biophotonics and Immune Responses IX
Wei R. Chen, Editor(s)

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