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Proceedings Paper

Hyperspectral imaging via spectral interferometric polarised coherent anti-Stokes Raman scattering
Author(s): Brad Littleton; Thomas Kavanagh; Frederic Festy; David Richards
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Paper Abstract

We have developed a new method for quantitative broadband CARS spectral imaging, which uses passive polarisation optics combined with spectrally-resolved balanced homodyne detection to remove the non-resonant background (NRB) in a single exposure. Independent measurement of the Stokes spectrum is not required for NRB removal, and the resulting spectra are amplified by the non-resonant response, vary linearly with concentration, and can be directly related to polarized spontaneous Raman spectra. The technique has relaxed requirements on spectral phase and instrument stability, is suitable for any laser system capable of generating CARS, and has been successfully applied to rapid hyperspectral Raman imaging.

Paper Details

Date Published: 28 February 2014
PDF: 10 pages
Proc. SPIE 8948, Multiphoton Microscopy in the Biomedical Sciences XIV, 89482T (28 February 2014); doi: 10.1117/12.2040940
Show Author Affiliations
Brad Littleton, King's College London (United Kingdom)
Thomas Kavanagh, King's College London (United Kingdom)
Frederic Festy, King's College London (United Kingdom)
David Richards, King's College London (United Kingdom)

Published in SPIE Proceedings Vol. 8948:
Multiphoton Microscopy in the Biomedical Sciences XIV
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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