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Proceedings Paper

Photothermal bleaching and recovery analysis in photoacoustic microscopy
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Paper Abstract

A novel method – photoacoustic recovery after photothermal bleaching (PRAP) – is proposed and implemented to study particle dynamics and medium properties at the micron scale via photoacoustic imaging. PRAP is an intuitive way to visualize as well as quantify dynamic processes in many kinds of media. We demonstrate PRAP first in a phantom study, and then in live cells. PRAP provides high signal-to-noise ratio imaging with minimal bleaching-induced artifacts during the recovery stage, ideal for monitoring the diffusive and kinetic phenomena inside a cell.

Paper Details

Date Published: 3 March 2014
PDF: 5 pages
Proc. SPIE 8943, Photons Plus Ultrasound: Imaging and Sensing 2014, 89435Q (3 March 2014); doi: 10.1117/12.2040936
Show Author Affiliations
Chiye Li, Washington Univ. in St. Louis (United States)
Chi Zhang, Washington Univ. in St. Louis (United States)
Liang Gao, Washington Univ. in St. Louis (United States)
Alejandro Garcia-Uribe, Washington Univ. in St. Louis (United States)
Lihong V. Wang, Washington Univ. in St. Louis (United States)


Published in SPIE Proceedings Vol. 8943:
Photons Plus Ultrasound: Imaging and Sensing 2014
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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