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Proceedings Paper

Optical fiber-based photomechanical molecular delivery system
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Paper Abstract

Molecular delivery based on nanosecond pulsed laser-induced photomechanical waves (PMWs) enables endoscopic application by using an optical fiber for laser transmission. In our previous fiber system, a laser target, which was a black natural rubber film as a laser absorbing material covered with an optically transparent polyethylene terephthalate disk to confine the laser-induced plasma, was attached to the output end of a 1 mm core diameter quartz fiber. There were two problems in that system: 1) the outer diameter was large (~2.7 mm) and 2) available peak pressure rapidly decreased with increasing pulse number. In this study, we developed a new fiber delivery system to overcome these problems. As a laser absorbing material, we used a cap-type silicone rubber containing carbon black, into which the fiber output end can simply be inserted. The fiber end surface works to confine the laser-induced plasma. The outer diameter of the fiber system was reduced to ~1.4 mm. At an output laser fluence of 1.2 J/cm2, peak pressure of the first PMW pulse exceeded ~40 MPa. With successive 10 laser pulses, decreasing rate of the peak pressure was 22%, which was considerably lower than that with the previous fiber system (82%), enabling generation of at least successive 30 pulses of PMW with the same cap-type target. With this fiber system, we attempted transfer of plasmid DNA encoding EGFP (enhanced green fluorescence protein) to the rat skin as a test tissue in vivo, showing site-selective efficient gene expression.

Paper Details

Date Published: 20 February 2014
PDF: 6 pages
Proc. SPIE 8938, Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIV, 89381J (20 February 2014); doi: 10.1117/12.2040904
Show Author Affiliations
Koki Nakano, Tokyo Univ. of Agriculture and Technology (Japan)
Shunichi Sato, National Defense Medical College Research Institute (Japan)
Satoko Kawauchi, National Defense Medical College Research Institute (Japan)
Hiroshi Ashida, National Defense Medical College Research Institute (Japan)
Izumi Nishidate, Tokyo Univ. of Agriculture and Technology (Japan)


Published in SPIE Proceedings Vol. 8938:
Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications XIV
Israel Gannot, Editor(s)

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