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Proceedings Paper

DNA-Aptamer optical biosensors based on a LPG-SPR optical fiber platform for point-of-care diagnostic
Author(s): L. Coelho; R. B. Queirós; J. L. Santos; M. Cristina L. Martins; D. Viegas; P. A. S. Jorge
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Paper Abstract

Surface Plasmon Resonance (SPR) is the base for some of the most sensitive label free optical fiber biosensors. However, most solutions presented to date require the use of fragile fiber optic structure such as adiabatic tapers or side polished fibers. On the other hand, long-period fiber gratings (LPG) present themselves as an interesting solution to attain an evanescent wave refractive index sensor platform while preserving the optical fiber integrity. The combination of these two approaches constitute a powerful platform that can potentially reach the highest sensitivities as it was recently demonstrated by detailed theoretical study [1, 2]. In this work, a LPG-SPR platform is explored in different configurations (metal coating between two LPG – symmetric and asymmetric) operating in the telecom band (around 1550 nm). For this purpose LPGs with period of 396 μm are combined with tailor made metallic thin films. In particular, the sensing regions were coated with 2 nm of chromium to improve the adhesion to the fiber and 16 nm of gold followed by a 100 nm thick layer of TiO2 dielectric material strategically chosen to attain plasmon resonance in the desired wavelength range. The obtained refractometric platforms were then validated as a biosensor. For this purpose the detection of thrombin using an aptamer based probe was used as a model system for protein detection. The surface of the sensing fibers were cleaned with isopropanol and dried with N2 and then the aminated thrombin aptamer (5’-[NH2]- GGTTGGTGTGGTTGG-3’) was immobilized by physisorption using Poly-L-Lysine (PLL) as cationic polymer. Preliminary results indicate the viability of the LPFG-SPR-APTAMER as a flexible platforms point of care diagnostic biosensors.

Paper Details

Date Published: 3 March 2014
PDF: 7 pages
Proc. SPIE 8957, Plasmonics in Biology and Medicine XI, 89570K (3 March 2014); doi: 10.1117/12.2040892
Show Author Affiliations
L. Coelho, INESC Porto (Portugal)
Univ. do Porto (Portugal)
R. B. Queirós, INESC Porto (Portugal)
J. L. Santos, INESC Porto (Portugal)
Univ. do Porto (Portugal)
M. Cristina L. Martins, Instituto de Engenharia Biomédica (Portugal)
D. Viegas, INESC Porto (Portugal)
P. A. S. Jorge, INESC Porto (Portugal)


Published in SPIE Proceedings Vol. 8957:
Plasmonics in Biology and Medicine XI
Tuan Vo-Dinh; Joseph R. Lakowicz, Editor(s)

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