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Proceedings Paper

Validation of temperature-modulated fluorescence tomography in vivo
Author(s): Tiffany C. Kwong; Farouk Nouizi; Yuting Lin; Rushi Rajyaguru; Trinh Nguyen; Lara Alptekin; Uma Sampathkumaran; Yue Zhu; Shaaz Ahmed; Gultekin Gulsen
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Paper Abstract

To overcome the strong scattering in biological tissue that has long afflicted fluorescence tomography, we have developed a novel technique, "temperature-modulated fluorescence tomography" (TM-FT) to combine the sensitivity of fluorescence imaging with focused ultrasound resolution. TM-FT relies on two key elements: temperature sensitive ICG loaded pluronic nanocapsules we termed ThermoDots and high intensity focused ultrasound (HIFU). TM-FT localizes the position of the fluorescent ThermoDots by irradiating and scanning a HIFU beam across the tissue while conventional fluorescence tomography measurements are acquired. The HIFU beam produces a local hot spot, in which the temperature suddenly increases changing the quantum efficiency of the ThermoDots. The small size of the focal spot (~1 mm) up to a depth of 6 cm, allows imaging the distribution of these temperature sensitive agents with not only high spatial resolution but also high quantitative accuracy in deep tissue using a proper image reconstruction algorithm. Previously we have demonstrated this technique with a phantom study with ThermoDots sensitive in the 20-25°C range. We recently optimized the ThermoDots for physiological temperatures. In this work, we will demonstrate a new HIFU scanning method which is optimized for in vivo studies. The performance of the system is tested using a phantom that resembles a small animal bearing a small tumor targeted by ThermoDots.

Paper Details

Date Published: 17 February 2014
PDF: 7 pages
Proc. SPIE 8937, Multimodal Biomedical Imaging IX, 89370H (17 February 2014); doi: 10.1117/12.2040878
Show Author Affiliations
Tiffany C. Kwong, Univ. of California, Irvine (United States)
Farouk Nouizi, Univ. of California, Irvine (United States)
Yuting Lin, Univ. of California, Irvine (United States)
Rushi Rajyaguru, Univ. of California, Irvine (United States)
Trinh Nguyen, Univ. of California, Irvine (United States)
Lara Alptekin, Univ. of California, Irvine (United States)
Uma Sampathkumaran, InnoSense LLC (United States)
Yue Zhu, InnoSense LLC (United States)
Shaaz Ahmed, InnoSense LLC (United States)
Gultekin Gulsen, Univ. of California, Irvine (United States)

Published in SPIE Proceedings Vol. 8937:
Multimodal Biomedical Imaging IX
Fred S. Azar; Xavier Intes, Editor(s)

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