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Proceedings Paper

Intracellular light-induced release of signaling molecules from gold-coated liposomes
Author(s): Gabriel V. Orsinger; Joshua D. Williams; Marek Romanowski
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Paper Abstract

The combination of laser light and composite nanovesicles enables unique opportunities for precise delivery to, and ondemand release of molecular compounds within, single cells at high spatiotemporal resolution. Here, we demonstrate precise delivery and intracellular release of molecules from gold-coated liposomes via near infrared (NIR) light. The plasmon resonant gold shell provides a light-sensitive trigger for on-demand content release from thermosensitive liposomes. Two demonstrations of intracellular delivery and release from gold-coated liposomes are presented here. The first example uses microinjection to preload gold-coated liposomes into a single cell, followed by exposure to onresonant NIR laser light to trigger release of a fluorescent nuclear dye intracellularly. In the second delivery and release demonstration, gold-coated liposomes encapsulating inositol trisphosphate (IP3), a ubiquitous secondary messenger in cell signaling cascades, passively accumulate within cells via endocytosis. Exposure to on-resonant NIR laser wavelength of light induces rapid release of IP3 from the intracellular liposomes and subsequent activation of Ca2+ signaling at a single cell, monitored by changes in fluorescence intensity of a Ca 2+-sensitive dye.

Paper Details

Date Published: 24 March 2014
PDF: 7 pages
Proc. SPIE 8955, Colloidal Nanoparticles for Biomedical Applications IX, 89551G (24 March 2014); doi: 10.1117/12.2040854
Show Author Affiliations
Gabriel V. Orsinger, The Univ. of Arizona (United States)
Joshua D. Williams, The Univ. of Arizona (United States)
The Univ. of Arizona Cancer Ctr. (United States)
Marek Romanowski, The Univ. of Arizona (United States)

Published in SPIE Proceedings Vol. 8955:
Colloidal Nanoparticles for Biomedical Applications IX
Wolfgang J. Parak; Marek Osinski; Kenji I. Yamamoto, Editor(s)

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