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Proceedings Paper

Evaluation of the Photodynamic Therapy effect using a tumor model in Chorioallantoic Membrane with Melanoma cells
Author(s): Hilde Harb Buzzá; Layla Pires; Vanderlei S. Bagnato; Cristina Kurachi
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Paper Abstract

Photodynamic Therapy (PDT) is a type of cancer treatment that is based on the interaction of light (with specific wavelength), a photosensitizing agent and molecular oxygen. The photosensitizer (PS) is activated by light and reacts with oxygen resulting in the production of singlet oxygen that is highly reactive and responsible for the cell death. The Chick Chorioallantoic Membrane (CAM) model is a transparent membrane that allows visualization and evaluation of blood vessels and structural changes, where a tumor model was developed. Two induction tumor models were investigated: tumor biopsy or cell culture. It was used a murine melanoma cell B16F10 in culture and a biopsy from a xenograft tumor in hairless mouse. Two PS were tested: Photodithazine® and Photogem®, a chlorine and porphyrin compounds, respectively. Using intravenous administration, the light-drug interval was of 30 minutes, 1 and 3 hours. Illumination was performed at 630 nm and 660 nm, and the vascular and tumor response was monitored and analyzed. The PS distribution was checked with confocal microscopy. This model can be useful to study several parameters of PDT and the effect of this therapy in the cancer treatment since it allows direct visualization of its effects.

Paper Details

Date Published: 14 April 2014
PDF: 7 pages
Proc. SPIE 8931, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXIII, 89310B (14 April 2014); doi: 10.1117/12.2040714
Show Author Affiliations
Hilde Harb Buzzá, Univ. de São Paulo (Brazil)
Layla Pires, Univ. de São Paulo (Brazil)
Vanderlei S. Bagnato, Univ. de São Paulo (Brazil)
Cristina Kurachi, Univ. de São Paulo (Brazil)

Published in SPIE Proceedings Vol. 8931:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXIII
David H. Kessel; Tayyaba Hasan, Editor(s)

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