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Proceedings Paper

Raman tweezers in microfluidic systems for analysis and sorting of living cells
Author(s): Zdenĕk Pilát; Jan Ježek; Jan Kaňka; Pavel Zemánek
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Paper Abstract

We have devised an analytical and sorting system combining optical trapping with Raman spectroscopy in microfluidic environment in order to identify and sort biological objects, such as living cells of various prokaryotic and eukaryotic organisms. Our main objective was to create a robust and universal platform for non-contact sorting of microobjects based on their Raman spectral properties. This approach allowed us to collect information about the chemical composition of the objects, such as the presence and composition of lipids, proteins, or nucleic acids without using artificial chemical probes such as fluorescent markers. The non-destructive and non-contact nature of this optical analysis and manipulation allowed us to separate individual living cells of our interest in a sterile environment and provided the possibility to cultivate the selected cells for further experiments. We used differently treated cells of algae to test and demonstrate the function of our analytical and sorting system. The devised system could find its use in many medical, biotechnological, and biological applications.

Paper Details

Date Published: 4 March 2014
PDF: 9 pages
Proc. SPIE 8947, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII, 89471M (4 March 2014); doi: 10.1117/12.2040631
Show Author Affiliations
Zdenĕk Pilát, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Jan Ježek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Jan Kaňka, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)
Pavel Zemánek, Institute of Scientific Instruments of the ASCR, v.v.i. (Czech Republic)


Published in SPIE Proceedings Vol. 8947:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XII
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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