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Proceedings Paper

PEGylation of Concanavalin A to decrease nonspecific interactions in a fluorescent glucose sensor
Author(s): Alexander A. Abraham; Brian M. Cummins; Andrea K. Locke; Melissa A. Grunlan; Gerard L. Coté
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Paper Abstract

The ability of people with diabetes to both monitor and regulate blood sugar levels is limited by the conventional “finger-prick” test that provides intermittent, single point measurements. Toward the development of a continuous glucose monitoring (CGM) system, the lectin, Concanavalin A (ConA), has been utilized as a component in a Förster resonance energy transfer (FRET), competitive glucose binding assay. Recently, to avoid reversibility problems associated with ConA aggregation, a suitable competing ligand labeled with 8-aminopyrene-1,3,6-trisulfonic acid trisodium salt (APTS) has been engineered. However, its ability to function as part of a glucose sensing assay is compromised due to the negative charge (at physiological pH) of native ConA that gives rise to non-specific binding with other ConA groups as well as with electrostatically charged assay-delivery carriers. To minimize these undesirable interactions, we have conjugated ConA with monomethoxy-poly(ethylene glycol) (mPEG) (i.e. “PEGylation”). In this preliminary research, fluorescently-labeled ConA was successfully PEGylated with mPEG-Nhydroxylsuccinimide( succinimidyl carbonate) (mPEG-NHS(SC)). The FRET response of APTS-labeled competing ligand (donor) conveyed an increase in the fluorescence intensity with increasing glucose concentrations.

Paper Details

Date Published: 28 February 2014
PDF: 5 pages
Proc. SPIE 8951, Optical Diagnostics and Sensing XIV: Toward Point-of-Care Diagnostics, 895112 (28 February 2014); doi: 10.1117/12.2040457
Show Author Affiliations
Alexander A. Abraham, Texas A&M Univ. (United States)
Brian M. Cummins, Texas A&M Univ. (United States)
Andrea K. Locke, Texas A&M Univ. (United States)
Melissa A. Grunlan, Texas A&M Univ. (United States)
Gerard L. Coté, Texas A&M Univ. (United States)

Published in SPIE Proceedings Vol. 8951:
Optical Diagnostics and Sensing XIV: Toward Point-of-Care Diagnostics
Gerard L. Coté, Editor(s)

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