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Proceedings Paper

Application of SERS spectroscopy for detection of trace components in urinary deposits
Author(s): Milda Pucetaite; Martynas Velicka; Sandra Tamosaityte; Valdas Sablinskas
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Paper Abstract

Surface-enhanced Raman scattering (SERS) spectroscopy can be a useful tool in regard to disease diagnosis and prevention. Advantage of SERS over conventional Raman spectroscopy is its significantly increased signal (up to factor of 106-108) which allows detection of trace amounts of substances in the sample. So far, this technique is successfully used for analysis of food, pieces of art and various biochemical/biomedical samples. In this work, we survey the possibility of applying SERS spectroscopy for detection of trace components in urinary deposits. Early discovery together with the identification of the exact chemical composition of urinary sediments could be crucial for taking appropriate preventive measures that inhibit kidney stone formation or growth processes. In this initial study, SERS spectra (excitation wavelength - 1064 nm) of main components of urinary deposits (calcium oxalate, uric acid, cystine, etc.) were recorded by using silver (Ag) colloid. Spectra of 10-3-10-5 M solutions were obtained. While no/small Raman signal was detected without the Ag colloid, characteristic peaks of the substances could be clearly separated in the SERS spectra. This suggests that even small amounts of the components could be detected and taken into account while determining the type of kidney stone forming in the urinary system. We found for the first time that trace amounts of components constituting urinary deposits could be detected by SERS spectroscopy. In the future study, the analysis of centrifuged urine samples will be carried out.

Paper Details

Date Published: 3 March 2014
PDF: 8 pages
Proc. SPIE 8957, Plasmonics in Biology and Medicine XI, 895708 (3 March 2014); doi: 10.1117/12.2040386
Show Author Affiliations
Milda Pucetaite, Vilnius Univ. (Lithuania)
Martynas Velicka, Vilnius Univ. (Lithuania)
Sandra Tamosaityte, Vilnius Univ. (Lithuania)
Valdas Sablinskas, Vilnius Univ. (Lithuania)


Published in SPIE Proceedings Vol. 8957:
Plasmonics in Biology and Medicine XI
Tuan Vo-Dinh; Joseph R. Lakowicz, Editor(s)

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