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Proceedings Paper

Optical properties of cells with melanin
Author(s): Barukh Rohde; Israel Coats; James Krueger; Dan Gareau
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Paper Abstract

The optical properties of pigmented lesions have been studied using diffuse reflectance spectroscopy in a noninvasive configuration on optically thick samples such as skin in vivo. However, it is difficult to un-mix the effects of absorption and scattering with diffuse reflectance spectroscopy techniques due to the complex anatomical distributions of absorbing and scattering biomolecules. We present a device and technique that enables absorption and scattering measurements of tissue volumes much smaller than the optical mean-free path. Because these measurements are taken on fresh-frozen sections, they are direct measurements of the optical properties of tissue, albeit in a different hydration state than in vivo tissue. Our results on lesions from 20 patients including melanomas and nevi show the absorption spectrum of melanin in melanocytes and basal keratinocytes. Our samples consisted of fresh frozen sections that were unstained. Fitting the spectrum as an exponential decay between 500 and 1100 nm [mua = A*exp(-B*(lambda-C)) + D], we report on the fit parameters of and their variation due to biological heterogeneity as A = 4.20e4 +/- 1.57e5 [1/cm], B = 4.57e-3 +/- 1.62e-3 [1/nm], C = 210 +/- 510 [nm] , D = 613 +/- 534 [1/cm]. The variability in these results is likely due to highly heterogeneous distributions of eumelanin and pheomelanin.

Paper Details

Date Published: 26 February 2014
PDF: 9 pages
Proc. SPIE 8941, Optical Interactions with Tissue and Cells XXV; and Terahertz for Biomedical Applications, 89410Y (26 February 2014); doi: 10.1117/12.2040361
Show Author Affiliations
Barukh Rohde, The Rockefeller Univ. (United States)
Hunter College, CUNY (United States)
Israel Coats, The Rockefeller Univ. (United States)
James Krueger, The Rockefeller Univ. (United States)
Dan Gareau, The Rockefeller Univ. (United States)


Published in SPIE Proceedings Vol. 8941:
Optical Interactions with Tissue and Cells XXV; and Terahertz for Biomedical Applications
E. Duco Jansen; Robert J. Thomas; Gerald J. Wilmink; Bennett L. Ibey, Editor(s)

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