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Proceedings Paper

Parameter determination for BPD mediated vascularPDT
Author(s): Michele M. Kim; Baochang Liu; Joann Miller; Theresa M. Busch; Timothy C. Zhu
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Paper Abstract

The cell killing mechanism of benzoporphyrin derivative monoacid ring A (BPD) is known to be predominantly apoptotic or vascular, depending on the drug-light interval (DLI). With a 3 hour DLI, necrosis develops secondary to tumor cell damage, while with a 15 minute DLI, necrosis results from treatment-created vascular damage. The purpose of this study is to examine if the different mechanisms of cell death will affect the photochemical parameters for the macroscopic singlet oxygen model. Using the RIF model of murine fibrosarcoma, we determined the four photochemical parameters (see manuscript) and the threshold singlet oxygen dose for BPD-mediated PDT through evaluation of the extent of tumor necrosis as a function of PDT fluence rate and total fluence. Mice were treated with a linear source at fluence rates from 12-150 mW/cm and total fluences from 24-135 J/cm. BPD was administered at 1mg/kg with a 15 minute DLI, followed by light delivery at 690nm. Tumors were excised at 24 hours after PDT and necrosis was analyzed via HE staining. The in-vivo BPD drug concentration is determined to be in the range of 0.05-0.30 μM. The determination of these parameters specific for BPD and the 15 minute DLI provides necessary data for predicting treatment outcome in clinical BPD-mediated PDT. Photochemical parameters will be compared between 1mg/kg DLI 3 hours and 1mg/kg DLI 15 minutes.

Paper Details

Date Published: 14 April 2014
PDF: 6 pages
Proc. SPIE 8931, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXIII, 89311D (14 April 2014); doi: 10.1117/12.2040305
Show Author Affiliations
Michele M. Kim, Univ. of Pennsylvania (United States)
Baochang Liu, Univ. of Pennsylvania (United States)
Joann Miller, Univ. of Pennsylvania (United States)
Theresa M. Busch, Univ. of Pennsylvania (United States)
Timothy C. Zhu, Univ. of Pennsylvania (United States)

Published in SPIE Proceedings Vol. 8931:
Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXIII
David H. Kessel; Tayyaba Hasan, Editor(s)

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