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Proceedings Paper

Photodynamic therapy improves the ultraviolet-irradiated hairless mice skin
Author(s): Ana Elisa S. Jorge; Michael R. Hamblin; Nivaldo A. Parizotto; Cristina Kurachi; Vanderlei S. Bagnato
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Paper Abstract

Chronic exposure to ultraviolet (UV) sunlight causes premature skin aging. In light of this fact, photodynamic therapy (PDT) is an emerging modality for treating cancer and other skin conditions, however its response on photoaged skin has not been fully illustrated by means of histopathology. For this reason, the aim of this study was analyze whether PDT can play a role on a mouse model of photoaging. Hence, SKH-1 hairless mice were randomly allocated in two groups, UV and UV/PDT. The mice were daily exposed to an UV light source (280-400 nm: peak at 350 nm) for 8 weeks followed by a single PDT session using 20% 5-aminolevulinic acid (ALA) topically. After the proper photosensitizer accumulation within the tissue, a non-coherent red (635 nm) light was performed and, after 14 days, skin samples were excised and processed for light microscopy, and their sections were stained with hematoxylin-eosin (HE) and Masson’s Trichrome. As a result, we observed a substantial epidermal thickening and an improvement in dermal collagen density by deposition of new collagen fibers on UV/PDT group. These findings strongly indicate epidermal and dermal restoration, and consequently skin restoration. In conclusion, this study provides suitable evidences that PDT improves the UV-irradiated hairless mice skin, supporting this technique as an efficient treatment for photoaged skin.

Paper Details

Date Published: 4 March 2014
PDF: 6 pages
Proc. SPIE 8926, Photonic Therapeutics and Diagnostics X, 89260N (4 March 2014); doi: 10.1117/12.2040297
Show Author Affiliations
Ana Elisa S. Jorge, Univ. de São Paulo (Brazil)
Wellman Ctr. for Photomedicine, Massachusetts General Hospital (United States)
Michael R. Hamblin, Wellman Ctr. for Photomedicine, Massachusetts General Hospital (United States)
Harvard Medical School (United States)
Harvard-MIT Division of Health Sciences and Technology (United States)
Nivaldo A. Parizotto, Univ. Federal de São Carlos (Brazil)
Cristina Kurachi, Univ. de São Paulo (Brazil)
Vanderlei S. Bagnato, Univ. de São Paulo (Brazil)


Published in SPIE Proceedings Vol. 8926:
Photonic Therapeutics and Diagnostics X
Bernard Choi; Hyun Wook Kang; Brian J. F. Wong; Guillermo J. Tearney; Andreas Mandelis; Nikiforos Kollias; Kenton W. Gregory; Justus F. Ilgner; Haishan Zeng; Laura Marcu, Editor(s)

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