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Proceedings Paper

Mediating the potent ROS toxicity of acrolein in neurons with silica nanoparticles and a natural product approach
Author(s): Désirée White-Schenk; Riyi Shi; James F. Leary
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Paper Abstract

Acrolein, a very reactive aldehyde, is a culprit in the biochemical cascade after primary, mechanical spinal cord injury (SCI), which leads to the destruction of tissue initially unharmed, referred to as “secondary injury”. Additionally, in models of multiple sclerosis (MS) and some clinical research, acrolein levels are significantly increased. Due to its ability to make more copies of itself in the presence of tissue via lipid peroxidation, researchers believe that acrolein plays a role in the increased destruction of the central nervous system in both SCI and MS. Hydralazine, an FDAapproved hypotensive drug, has been shown to scavenge acrolein, but its side effects and short half life at the appropriate dose for acrolein scavenging must be improved for beneficial clinical translation. Therefore, a nanomedical approach has been designed using silica nanoparticles as a porous delivery vehicle hydralazine. The silica particles are formed in a one-step method that incorporates poly(ethylene) glycol (PEG), a stealth molecule, directly onto the nanoparticles. As an additional avenue for study, a natural product in green tea, epigallocatechin gallate (EGCG), has been explored for its ability to react with acrolein, disabling its reactive capabilities. Upon demonstration of attenuating acrolein, EGCG's delivery may also be improved using the nanomedical approach. The current work exposes the potential of using silica nanoparticles as a delivery vehicle and EGCG's antioxidant capabilities in B35 neuroblastoma cells exposed to acrolein. We also measure nanotoxicity to individual rat neurons using high-throughput image scanning cytometry.

Paper Details

Date Published: 5 March 2014
PDF: 11 pages
Proc. SPIE 8956, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VI, 89560C (5 March 2014); doi: 10.1117/12.2040190
Show Author Affiliations
Désirée White-Schenk, Birck Nanotechnology Ctr., Purdue Univ. (United States)
Weldon School of Biomedical Engineering, Purdue Univ. (United States)
Riyi Shi, School of Veterinary Medicine, Purdue Univ. (United States)
Weldon School of Biomedical Engineering, Purdue Univ. (United States)
James F. Leary, Birck Nanotechnology Ctr., Purdue Univ. (United States)
School of Veterinary Medicine, Purdue Univ. (United States)
Weldon School of Biomedical Engineering, Purdue Univ. (United States)


Published in SPIE Proceedings Vol. 8956:
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications VI
Samuel Achilefu; Ramesh Raghavachari, Editor(s)

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