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Proceedings Paper

Label-free assay for the detection of glucose mediated by the effects of narrowband absorption on quantum dot photoluminescence
Author(s): Saara A. Khan; Gennifer T. Smith; Audrey K. Ellerbee
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Paper Abstract

We present a novel strategy for label-free detection of glucose based on CdSe/ZnS core/shell quantum dots (QDs). We exploit the concentration-dependent, narrowband absorption of the hexokinase-glucose 6-phosphate dehydrogenase enzymatic assay to selectively filter a 365-nm excitation source, leading to a proportional decrease in the photoluminescence intensity of the QDs. The visible wavelength emission of the QDs enables quantitative readout using standard visible detectors (e.g., CCD). Experimental results show highly linear QD photoluminescence over the clinically relevant glucose concentration range of 1-25mM, in excellent agreement with detection methods demonstrated by others. The method has a demonstrated limit of detection of 3.5μM, also on par with the best proposed methods. A significant advantage of our strategy is the complete elimination of QDs as a consumable. In contrast with other methods of QD-based measurement of glucose, our system does not require the glucose solution to be mixed with the QDs, thereby decreasing its overall cost and making it an ideal strategy for point-of-care detection of glucose in low-resource areas. Furthermore, readout can be accomplished with low-cost, portable detectors such as cellular phones, eliminating the need for expensive and bulky spectrophotometers to output quantitative information. The general strategy we present is useful for other biosensing applications involving chemistries with unique absorption peaks falling within the excitation band of available QDs.

Paper Details

Date Published: 5 March 2014
PDF: 7 pages
Proc. SPIE 8933, Frontiers in Biological Detection: From Nanosensors to Systems VI, 89330A (5 March 2014); doi: 10.1117/12.2039963
Show Author Affiliations
Saara A. Khan, Stanford Univ. (United States)
Gennifer T. Smith, Stanford Univ. (United States)
Audrey K. Ellerbee, Stanford Univ. (United States)

Published in SPIE Proceedings Vol. 8933:
Frontiers in Biological Detection: From Nanosensors to Systems VI
Benjamin L. Miller; Philippe M. Fauchet; Brian T. Cunningham, Editor(s)

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